Key Product Details

Species Reactivity

Human, Bovine (Negative), Canine (Negative), Primate, Rat (Negative), Sheep (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # QBEnd/10

Format

Azide and BSA Free
View all CD34 Primary Antibodies »

Product Specifications

Immunogen

Detergent solubilized vesicular suspension prepared from human term placenta

Reactivity Notes

Cynomolgus Monkey, Rhesus Monkey

Localization

Cell surface

Marker

Hematopoietic Stem Cell & Endothelial Marker

Specificity

This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse CD34 Antibody (QBEnd/10) - Azide and BSA Free (NBP2-32932) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-CD34 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD34 Antibody (QBEnd/10) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10). Image using the standard format of this product.
Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Human placental tissue. Antigen retrieval at pH 9. IF by Cy3 conjugate. Image from verified customer review.
Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713]

Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD34 MAb (QBEnd/10) Image using the standard format of this product.
CD34 Antibody (QBEnd/10) - Azide and BSA Free

CD34 (QBEnd/10) in U-2 OS Human Cell Line.

CD34 (QBEnd/10) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-CD34 (QBEnd/10) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-34713AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for CD34 Antibody (QBEnd/10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Frozen

0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Immunoprecipitation

0.5-1ug/500ug protein lysate

Western Blot

0.25-0.5ug/ml
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Reviewed Applications

Read 1 review rated 4 using NBP2-34713 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD34

CD34, also known as Cluster of Differentiation 34, is a transmembrane phosphoglycoprotein that is primarily known for being a surface marker for hematopoietic stem cells (HSCs) and progenitor cells (1,2). The CD34 protein consists of a heavily glycosylated extracellular domain, a single-pass transmembrane helix, and a cytoplasmic tail with PDZ binding motifs (1,3). Human CD34 has two protein isoforms consisting of 385 amino acids (aa) for the canonical isoform and 328 aa for isoform 2 (3,4). The canonical CD34 isoform has calculated theoretical molecular weight of 40 kDa and an observed molecular weight of ~115 kDa (1,3,4). L-Selectin (CD62L) is the most common ligand for CD34, but it has also been shown to bind CrkL (1,3).

CD34 has commonly been used as a marker for the diagnosis and classification of various diseases and pathologies including leukemia and solitary fibrous tumor (SFT) (2,5). In terms of immunohistochemistry and histopathology, CD34 has been the most common marker for SFT and is expressed in ~79% of cases (5). In addition to its use as a cell marker, CD34-postive (CD34+) hematopoietic stem cells have been used therapeutically in patients following radiation or chemotherapy due to their regenerative potential (6). There are several clinical trials showing promising results for CD34+ cell therapy for cardiovascular diseases including heart failure, ischemia, dilated cardiomyopathy, acute myocardial infarction, and angina (6). Besides hematopoietic lineages, CD34 is also expressed in non-hematopoietic cells including mesenchymal stem cells, endothelial cells and progenitors, fibrocytes, muscle satellite cells, and some cancer stem cells (1,3). While the clinical and cell therapy applications of CD34 as a cell marker is well documented, the function of CD34 is less understood but has been implicated in many cellular processes such as adhesion, proliferation, signal transduction, differentiation, and progenitor phenotype maintenance (1,3).

References

1. Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem cells (Dayton, Ohio), 32(6), 1380-1389. https://doi.org/10.1002/stem.1661

2. Krause, D. S., Fackler, M. J., Civin, C. I., & May, W. S. (1996). CD34: structure, biology, and clinical utility. Blood, 87(1), 1-13

3. Kapoor, S., Shenoy, S. P., & Bose, B. (2020). CD34 cells in somatic, regenerative and cancer stem cells: Developmental biology, cell therapy, and omics big data perspective. Journal of cellular biochemistry, 121(5-6), 3058-3069. https://doi.org/10.1002/jcb.29571

4. Uniprot (P28906)

5. Davanzo, B., Emerson, R. E., Lisy, M., Koniaris, L. G., & Kays, J. K. (2018). Solitary fibrous tumor. Translational gastroenterology and hepatology, 3, 94. https://doi.org/10.21037/tgh.2018.11.02

6. Prasad, M., Corban, M. T., Henry, T. D., Dietz, A. B., Lerman, L. O., & Lerman, A. (2020). Promise of autologous CD34+ stem/progenitor cell therapy for treatment of cardiovascular disease. Cardiovascular research, 116(8), 1424-1433. https://doi.org/10.1093/cvr/cvaa027

Alternate Names

CD34, HPCA1

Gene Symbol

CD34

Additional CD34 Products

Product Documents for CD34 Antibody (QBEnd/10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD34 Antibody (QBEnd/10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD34 Antibody (QBEnd/10) - Azide and BSA Free

Customer Reviews for CD34 Antibody (QBEnd/10) - Azide and BSA Free (1)

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Customer Images

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  • CD34 Antibody (QBEnd/10)
    Name: Christian Schuerch
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Placental tissue
    Species: Human
    Verified Customer | Posted 09/30/2018
    pH 9 antigen retrieval
    CD34 Antibody (QBEnd/10) - Azide and BSA Free NBP2-34713

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Protocols

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FAQs for CD34 Antibody (QBEnd/10) - Azide and BSA Free

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    • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
      A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
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