CD34 Antibody (SI16-01)

Novus Biologicals | Catalog # NBP2-67399

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SI16-01 expressed in HEK293
View all CD34 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CD34 aa 336-385 / 385. (SwissProt: P28906 Human; SwissProt: Q64314 Mouse; Unigene: 219720 Rat)

Localization

Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD34 Antibody (SI16-01)

Western Blot: CD34 Antibody (SI16-01) [NBP2-67399]

Western Blot: CD34 Antibody (SI16-01) [NBP2-67399]

Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate
Immunocytochemistry/ Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399]

Immunocytochemistry/ Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399]

CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg
Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399]

Immunocytochemistry/ Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399]

Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399]

Immunocytochemistry/ Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399]

Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399]

Immunocytochemistry/ Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399]

Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399]

Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for CD34 Antibody (SI16-01)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:200-1:400

Multiplex Immunofluorescence

1:1000

Western Blot

1:500-1:2000
Application Notes
IP: Use at an assay dependent concentration.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD34

CD34, also known as Cluster of Differentiation 34, is a transmembrane phosphoglycoprotein that is primarily known for being a surface marker for hematopoietic stem cells (HSCs) and progenitor cells (1,2). The CD34 protein consists of a heavily glycosylated extracellular domain, a single-pass transmembrane helix, and a cytoplasmic tail with PDZ binding motifs (1,3). Human CD34 has two protein isoforms consisting of 385 amino acids (aa) for the canonical isoform and 328 aa for isoform 2 (3,4). The canonical CD34 isoform has calculated theoretical molecular weight of 40 kDa and an observed molecular weight of ~115 kDa (1,3,4). L-Selectin (CD62L) is the most common ligand for CD34, but it has also been shown to bind CrkL (1,3).

CD34 has commonly been used as a marker for the diagnosis and classification of various diseases and pathologies including leukemia and solitary fibrous tumor (SFT) (2,5). In terms of immunohistochemistry and histopathology, CD34 has been the most common marker for SFT and is expressed in ~79% of cases (5). In addition to its use as a cell marker, CD34-postive (CD34+) hematopoietic stem cells have been used therapeutically in patients following radiation or chemotherapy due to their regenerative potential (6). There are several clinical trials showing promising results for CD34+ cell therapy for cardiovascular diseases including heart failure, ischemia, dilated cardiomyopathy, acute myocardial infarction, and angina (6). Besides hematopoietic lineages, CD34 is also expressed in non-hematopoietic cells including mesenchymal stem cells, endothelial cells and progenitors, fibrocytes, muscle satellite cells, and some cancer stem cells (1,3). While the clinical and cell therapy applications of CD34 as a cell marker is well documented, the function of CD34 is less understood but has been implicated in many cellular processes such as adhesion, proliferation, signal transduction, differentiation, and progenitor phenotype maintenance (1,3).

References

1. Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem cells (Dayton, Ohio), 32(6), 1380-1389. https://doi.org/10.1002/stem.1661

2. Krause, D. S., Fackler, M. J., Civin, C. I., & May, W. S. (1996). CD34: structure, biology, and clinical utility. Blood, 87(1), 1-13

3. Kapoor, S., Shenoy, S. P., & Bose, B. (2020). CD34 cells in somatic, regenerative and cancer stem cells: Developmental biology, cell therapy, and omics big data perspective. Journal of cellular biochemistry, 121(5-6), 3058-3069. https://doi.org/10.1002/jcb.29571

4. Uniprot (P28906)

5. Davanzo, B., Emerson, R. E., Lisy, M., Koniaris, L. G., & Kays, J. K. (2018). Solitary fibrous tumor. Translational gastroenterology and hepatology, 3, 94. https://doi.org/10.21037/tgh.2018.11.02

6. Prasad, M., Corban, M. T., Henry, T. D., Dietz, A. B., Lerman, L. O., & Lerman, A. (2020). Promise of autologous CD34+ stem/progenitor cell therapy for treatment of cardiovascular disease. Cardiovascular research, 116(8), 1424-1433. https://doi.org/10.1093/cvr/cvaa027

Alternate Names

CD34, HPCA1

Gene Symbol

CD34

Additional CD34 Products

Product Documents for CD34 Antibody (SI16-01)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD34 Antibody (SI16-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD34 Antibody (SI16-01)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD34 Antibody (SI16-01)

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  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

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