CD90/Thy1 Antibody (7E1B11) - BSA Free

Novus Biologicals | Catalog # NBP2-37330

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7E1B11

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human CD90/Thy1 (AA: 17-132) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD90/Thy1 Antibody (7E1B11) - BSA Free

Western Blot: CD90/Thy1 Antibody (7E1B11)BSA Free [NBP2-37330]

Western Blot: CD90/Thy1 Antibody (7E1B11)BSA Free [NBP2-37330]

Western Blot: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis using THY1 mouse mAb against T47D (1), HepG2 (2) and PC-12 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunocytochemistry/ Immunofluorescence: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330] - CD90/Thy1 antibody (7E1B11) in indirect immunofluorescence staining of primary human fibroblasts using a red-fluorescent secondary antibody. Nuclei stained with DAPI (blue). Adjacent epithelial tumor cells stain negative for CD90. Image from verified customer review.
Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - CD90 staining in human tonsil and aorta tissue. IHC-P image submitted by a verified customer review.
Western Blot: CD90/Thy1 Antibody (7E1B11)BSA Free [NBP2-37330]

Western Blot: CD90/Thy1 Antibody (7E1B11)BSA Free [NBP2-37330]

Western Blot: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis using THY1 mAb against human THY1 recombinant protein. (Expected MW is 38.5 kDa).
Western Blot: CD90/Thy1 Antibody (7E1B11)BSA Free [NBP2-37330]

Western Blot: CD90/Thy1 Antibody (7E1B11)BSA Free [NBP2-37330]

Western Blot: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis using THY1 mAb against HEK293 (1) and THY1 (AA: 17-132)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunocytochemistry/ Immunofluorescence: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Immunofluorescence analysis of Hela cells using THY1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis of endometrial cancer tissues using THY1 mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

Immunohistochemistry-Paraffin: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Analysis of cerebellum tissues using THY1 mouse mAb with DAB staining.
ELISA: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

ELISA: CD90/Thy1 Antibody (7E1B11) - BSA Free [NBP2-37330]

ELISA: CD90/Thy1 Antibody (7E1B11) [NBP2-37330] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for CD90/Thy1 Antibody (7E1B11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
Use in Flow cytometry reported in scientific literature (PMID: 30105667).

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-37330 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD90/Thy1

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.

Alternate Names

CD90, Thy1

Gene Symbol

THY1

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (7E1B11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD90/Thy1 Antibody (7E1B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD90/Thy1 Antibody (7E1B11) - BSA Free

Customer Reviews for CD90/Thy1 Antibody (7E1B11) - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Customer Images


Showing  1 - 2 of 2 reviews Showing All
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  • CD90/Thy1 Antibody (7E1B11) - BSA Free
    Name: Felix Oppel
    Application: Indirect Immunofluorescence
    Sample Tested: Cell culture
    Species: Human
    Verified Customer | Posted 05/03/2022
    CD90 antibody in indirect immunofluorescence staining of primary human fibroblasts using a red-fluorescent secondary antibody. Nuclei stained with DAPI (blue). Adjacent epithelial tumor cells stain negative for CD90.
    CD90/Thy1 Antibody (7E1B11) - BSA Free NBP2-37330
  • CD90/Thy1 Antibody (7E1B11)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil and Aorta tissue
    Species: Human
    Verified Customer | Posted 05/26/2021
    CD90 in human tonsil and aorta
    CD90/Thy1 Antibody (7E1B11) - BSA Free NBP2-37330

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Protocols

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FAQs for CD90/Thy1 Antibody (7E1B11) - BSA Free

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  • Q: We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A: I would recommend one of theses CD90 antibodies, as these have all been validated for use with dog/canine samples.

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