Human NT-3 Antibody

Catalog # Availability Size / Price Qty
AF-267-NA
AF-267-SP
Cell Proliferation Induced by NT‑3 and Neutralization by Human NT‑3 Antibody.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human NT-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NT-3 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) NT‑4 is observed, and less than 1% cross-reactivity with rhBDNF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NT-3
Tyr139-Thr257
Accession # P20783
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NT‑3 (Catalog # 267-N3)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize NT‑3-induced proliferation in BaF‑TrKB-BD mouse pro‑B cell line transfected with TrkB. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 20 ng/mL Recombinant Human NT‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Cell Proliferation Induced by NT‑3 and Neutralization by Human NT‑3 Antibody. View Larger

Cell Proliferation Induced by NT‑3 and Neutralization by Human NT‑3 Antibody. Recombinant Human NT‑3 (Catalog # 267-N3) stimulates proliferation in BaF‑TrKB‑BD mouse pro‑B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT‑3 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human NT‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-267-NA). The ND50 is typically 0.1-0.4 µg/mL.

Immunohistochemistry NT‑3 in Rat Brain. View Larger

NT‑3 in Rat Brain. NT‑3 was detected in perfusion fixed frozen sections of rat brain (Purkinje cells in the cerebellum) using 10 µg/mL Human NT‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑267‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NT-3

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

References
  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Neurotrophin 3
Entrez Gene IDs
4908 (Human)
Alternate Names
3-nucleotidase; NT3; NT-3; NTF3

Product Datasheets

Citation for Human NT-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
    Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR
    J. Mol. Cell. Cardiol., 2010;49(1):79-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neut

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human NT-3 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human NT-3 Antibody
By Anonymous on 07/22/2016
Application: IHC Sample Tested: Brain (cerebellum) tissue Species: Mouse

Human NT-3 Antibody
By Anonymous on 05/18/2016
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

over expression of proNT-3