Human IA-2/PTPRN Antibody

Catalog # Availability Size / Price Qty
MAB7906
MAB7906-SP
Detection of Human IA-2/PTPRN by Western Blot.
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Human IA-2/PTPRN Antibody Summary

Species Reactivity
Human
Specificity
Detects human IA-2/PTPRN in ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 815811
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IA-2/PTPRN
Val35-Arg575
Accession # Q16849
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human IA-2/PTPRN antibody by Western Blot. View Larger

Detection of Human IA-2/PTPRN by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IA-2/PTPRN at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IA-2/PTPRN antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

IA-2/PTPRN in Human Pancreas. IA-2/PTPRN was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human IA-2/PTPRN antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human IA-2/PTPRN by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.5 mg/mL. A specific band was detected for IA-2/PTPRN at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IA-2/PTPRN

IA-2 (insulinoma-assocated protein 2), also called PTPRN (protein tyrosine phosphatase-like N) and ICA512 (islet cell antigen 512), is a 130 kDa member of the receptor tyrosine phosphatase family of type I transmembrane enzymes. It is not active as a phosphatase but acts an autoantigen in type I (insulin-dependent) diabetes. It is mainly expressed in neuroendocrine cells types within pancreatic islets, adrenal medulla, pituitary, and the central nervous system, and is present within membranes of regulated secretory granules. It is induced by glucose and insulin in the pancreas and is thought to contribute to the growth of beta cells. Cleavage at a furin-like site at amino acid (aa) 448 creates a 60-66 kDa transmembrane fragment. Within the region used as an immunogen, human IA-2 shares 80% aa sequence identity with mouse and rat IA-2.

Long Name
Islet Cell Antigen 2/Protein Tyrosine Phosphatase Receptor-type N
Entrez Gene IDs
5798 (Human); 19275 (Mouse); 116660 (Rat)
Alternate Names
CA 512; EC 3.1.3.48; FLJ16131; IA2; IA-2; IA-2/PTP; ICA 512; ICA3; ICA512; islet cell antigen 2; Islet cell antigen 512; Islet cell autoantigen 3; protein tyrosine phosphatase, receptor type, N; protein tyrosine phosphatase-like N; PTP IA-2; PTPRN; receptor-type tyrosine-protein phosphatase-like N; R-PTP-N

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human IA-2/PTPRN Antibody

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Human IA-2/PTPRN Antibody
By Anonymous on 12/02/2021
Application: IHC Sample Tested: Lung adenocarcinoma tissue Species: Human