Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 815811
View all IA-2/PTPRN Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IA-2/PTPRN
Val35-Arg575
Accession # Q16849

Specificity

Detects human IA-2/PTPRN in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human IA-2/PTPRN Antibody

Detection of Human IA-2/PTPRN antibody by Western Blot.

Detection of Human IA-2/PTPRN by Western Blot.

Western blot shows lysates of human brain (hypothalamus) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IA-2/PTPRN at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IA-2/PTPRN antibody in Human Pancreas by Immunohistochemistry (IHC-P).

IA-2/PTPRN in Human Pancreas.

IA-2/PTPRN was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human IA-2/PTPRN antibody by Simple WesternTM.

Detection of Human IA-2/PTPRN by Simple WesternTM.

Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.5 mg/mL. A specific band was detected for IA-2/PTPRN at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human IA-2/PTPRN Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Simple Western

10 µg/mL
Sample: Human brain (hypothalamus) tissue

Western Blot

1 µg/mL
Sample: Human brain (hypothalamus) tissue and human brain (hippocampus) tissue

Reviewed Applications

Read 1 review rated 5 using MAB7906 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IA-2/PTPRN

IA-2 (insulinoma-assocated protein 2), also called PTPRN (protein tyrosine phosphatase-like N) and ICA512 (islet cell antigen 512), is a 130 kDa member of the receptor tyrosine phosphatase family of type I transmembrane enzymes. It is not active as a phosphatase but acts an autoantigen in type I (insulin-dependent) diabetes. It is mainly expressed in neuroendocrine cells types within pancreatic islets, adrenal medulla, pituitary, and the central nervous system, and is present within membranes of regulated secretory granules. It is induced by glucose and insulin in the pancreas and is thought to contribute to the growth of beta cells. Cleavage at a furin-like site at amino acid (aa) 448 creates a 60-66 kDa transmembrane fragment. Within the region used as an immunogen, human IA-2 shares 80% aa sequence identity with mouse and rat IA-2.

Long Name

Islet Cell Antigen 2/Protein Tyrosine Phosphatase Receptor-type N

Alternate Names

IA2, ICA512, PTPRN, R-PTP-N

Entrez Gene IDs

5798 (Human); 19275 (Mouse); 116660 (Rat)

Gene Symbol

PTPRN

UniProt

Q16849 (Human)

Additional IA-2/PTPRN Products

Product Documents for Human IA-2/PTPRN Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IA-2/PTPRN Antibody

For research use only

Customer Reviews for Human IA-2/PTPRN Antibody (1)

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  • Human IA-2/PTPRN Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung adenocarcinoma tissue
    Species: Human
    Verified Customer | Posted 12/02/2021
    Human IA-2/PTPRN Antibody MAB7906

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Protocols

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