Human IA-2/PTPRN Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IA-2/PTPRN in ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 815811
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IA-2/PTPRN
    Val35-Arg575
    Accession # Q16849
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IA-2/PTPRN by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IA-2/PTPRN at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
IA-2/PTPRN in Human Pancreas. IA-2/PTPRN was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human IA-2/PTPRN by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.5 mg/mL. A specific band was detected for IA-2/PTPRN at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IA-2/PTPRN Monoclonal Antibody (Catalog # MAB7906). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.       
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IA-2/PTPRN
IA-2 (insulinoma-assocated protein 2), also called PTPRN (protein tyrosine phosphatase-like N) and ICA512 (islet cell antigen 512), is a 130 kDa member of the receptor tyrosine phosphatase family of type I transmembrane enzymes. It is not active as a phosphatase but acts an autoantigen in type I (insulin-dependent) diabetes. It is mainly expressed in neuroendocrine cells types within pancreatic islets, adrenal medulla, pituitary, and the central nervous system, and is present within membranes of regulated secretory granules. It is induced by glucose and insulin in the pancreas and is thought to contribute to the growth of beta cells. Cleavage at a furin-like site at amino acid (aa) 448 creates a 60-66 kDa transmembrane fragment. Within the region used as an immunogen, human IA-2 shares 80% aa sequence identity with mouse and rat IA-2.
  • Long Name:
    Islet Cell Antigen 2/Protein Tyrosine Phosphatase Receptor-type N
  • Entrez Gene IDs:
    5798 (Human); 19275 (Mouse); 116660 (Rat)
  • Alternate Names:
    CA 512; EC 3.1.3.48; FLJ16131; IA2; IA-2; IA-2/PTP; ICA 512; ICA3; ICA512; islet cell antigen 2; Islet cell antigen 512; Islet cell autoantigen 3; protein tyrosine phosphatase, receptor type, N; protein tyrosine phosphatase-like N; PTP IA-2; PTPRN; receptor-type tyrosine-protein phosphatase-like N; R-PTP-N
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