Key Product Details

Species Reactivity

Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 156808
View all Caronte Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant chicken Caronte
Asp16-Asn272
Accession # NP_990154

Specificity

Detects chicken Caronte in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cerberus, rhDAN, or recombinant mouse Gremlin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Chicken Caronte Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Chicken Caronte Fc Chimera (Catalog # 1051-CA)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caronte

Chicken Caronte, the ortholog of mammalian Cerberus, is a member of the Cerberus/DAN family of secreted glycoproteins. There are at least 5 Dan Domain (DAND) family members including DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), Cerberus (DAND4), and Coco (DAND5). Caronte is expressed in the left paraxial mesoderm and is the molecule responsible for transducing left-sided positional information from the node to the periphery of the lateral plate mesoderm (LPM) during chicken embryonic development. Caronte functions as a BMP antagonist, inducing Nodal and downstream genes in the LPM by interfering with the repressive activity of bilaterally-produced BMPs. In addition, antagonism of BMPs by Caronte induces the expression of the Lefty gene in the midline. Caronte binds both BMP-4 and BMP-7 in immunoprecipitation experiments. Recombinant chicken Caronte preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and induce heart reversals in Xenopus embryos.

References

  1. Pearce, J. et al. (1999) Dev. Biol. 209:98.
  2. Yokouchi, Y. et al. (1999) Cell. 98:573.
  3. King, T. and N. Brown (1999) Nature 401:222.
  4. Ahu, L. et al. (1999) Curr. Biol. 9:931.
  5. Rodriguez-Esteban, C. et al. (1999) Nature 401:243.

Alternate Names

Chicken Caronte

Entrez Gene IDs

395623 (Chicken)

Gene Symbol

CER1

UniProt

NP_990154

Additional Caronte Products

Product Documents for Chicken Caronte Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Chicken Caronte Antibody

For research use only

Related Research Areas

BMP Family

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