Chicken Netrin-1 Antibody

Catalog # Availability Size / Price Qty
MAB128
MAB128-SP
Product Details
Citations (2)
FAQs
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Chicken Netrin-1 Antibody Summary

Species Reactivity
Chicken
Specificity
Detects chicken Netrin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 1% cross-reactivity with recombinant chicken Netrin-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 126518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant chicken Netrin-1
Gly26-Ala606
Accession # Q90922
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Chicken Netrin‑1 (Catalog # 128-N1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-1

Chicken Netrin-1 is the prototypical member of an ever-expanding, laminin-related family of axon-guidance molecules collectively referrred to as netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 606 amino acid (aa) protein precursor that has structural similarity to the N-terminus of the B2 or gamma ‑chain of laminin. It contains one 250 aa type VI globular domain, three type V (~55 aa) cysteine/glycine rich EGF repeats, and one unique 140 aa “C” domain that binds heparin. Chicken Netrin-1 shares 78% aa identity with chicken Netrin-2 and 86% aa identity with mouse and human Netrin-1. Although only two chicken netrins are known, the number of known mammalian netrins is increasing. Human and mouse Netrin-3/NTN-2L, and a mouse Netrin-4, that shares homology with the B1 or beta ‑chain of laminin, have been reported. Cells reported to express Netrin-1 in the embryo include cells of the spinal cord floor plate and somite, cells of the ganglionic eminence, and cells of the floor plate of the met- and caudal mesencephalon. In the adult, neurons of the thalamus, neocortex, and hippocampus, plus Schwann cells, osteoclasts and osteoblasts all reportedly produce Netrin-1. The DCC (deleted in colorectal cancer) gene product as well as the UNC5 family of receptors and the adenosine A2b receptor have been proposed to be functional receptors for Netrin-1.

References
  1. Serafini, T. et al. (1994) Cell 78:409.
  2. Kennedy, T.E. et al. (1994) Cell 78:425.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. MacLennan, A.J. et al. (1997) J. Neurosci. 17:5466.
  5. Koch, M. et al. (2000) J. Cell. Biol. 151:221.
  6. Corset, V. et al. (2000) Nature 407:747.
Entrez Gene IDs
9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

Citations for Chicken Netrin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Detailed analysis of chick optic fissure closure reveals Netrin-1 as an essential mediator of epithelial fusion
    Authors: Holly Hardy, James GD Prendergast, Aara Patel, Sunit Dutta, Violeta Trejo-Reveles, Hannah Kroeger et al.
    eLife
  2. Netrin-1 induces apoptosis in human cervical tumor cells via the TAp73alpha tumor suppressor.
    Authors: Roperch JP, El Ouadrani K, Hendrix A, Emami S, De Wever O, Melino G, Gespach C
    Cancer Res., 2008-10-15;68(20):8231-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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