cIAP-1/HIAP-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77193

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (92%), Porcine (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all cIAP-1/HIAP-2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP-1/HIAP-2. c-IAP antibody detects both c-IAP1 and c-IAP2. The immunogen is located within amino acids 540-590 of cIAP-1/HIAP-2.

Specificity

Human cIAP-1/HIAP-2 has 2 isoforms, including isoform 1 (618aa, 70kD) and isoform 2 (569aa, 64kD). Mouse cIAP-1/HIAP-2 has one isoform (612aa, 70kD) and Rat cIAP-1/HIAP-2 also has one isoform (589aa, 67kD). NBP1-77193 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for cIAP-1/HIAP-2 Antibody - BSA Free

Western Blot: cIAP-1/HIAP-2 AntibodyBSA Free [NBP1-77193]

Western Blot: cIAP-1/HIAP-2 AntibodyBSA Free [NBP1-77193]

Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Western Blot Validation in Human and Rat Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: cIAP NBP1-77193 (1 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: cIAP-1/HIAP-2 AntibodyBSA Free [NBP1-77193]

Western Blot: cIAP-1/HIAP-2 AntibodyBSA Free [NBP1-77193]

Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Analysis in human lung lysate with antibody at 1 (lane A), 2 (lane B), and 4 (lane C) ug/mL, respectively.
Western Blot: cIAP-1/HIAP-2 AntibodyBSA Free [NBP1-77193]

Western Blot: cIAP-1/HIAP-2 AntibodyBSA Free [NBP1-77193]

Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: cIAP NBP1-77193 (2 ug/mL), cIAP, competitor antibody, (1 ug/mL) and beta-actin NBP1-76692 (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: cIAP-1/HIAP-2 AntibodyBSA Free [NBP1-77193]

Western Blot: cIAP-1/HIAP-2 AntibodyBSA Free [NBP1-77193]

Western Blot: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: cIAP NBP1-77193 (2 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: cIAP-1/HIAP-2 Antibody [NBP1-77193] -

Immunocytochemistry/Immunofluorescence: cIAP-1/HIAP-2 Antibody [NBP1-77193] - Human Lung cells with cIAP-1/HIAP-2 antibody at 20ug/ml
cIAP-1/HIAP-2 Antibody - BSA Free

Immunohistochemistry: cIAP-1/HIAP-2 Antibody - BSA Free [NBP1-77193] -

Immunohistochemistry: cIAP-1/HIAP-2 Antibody - BSA Free [NBP1-77193] - Validation of cIAP-1/HIAP-2 in Human Lung Tissue. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP-1/HIAP-2 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for cIAP-1/HIAP-2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

10 ug/mL

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: cIAP-1/HIAP-2

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-alpha by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.

Long Name

Cellular Inhibitor of Apoptosis Protein 1

Alternate Names

BIRC2, cIAP1, HIAP-2, MIHB

Entrez Gene IDs

329 (Human); 11797 (Mouse)

Gene Symbol

BIRC2

UniProt

Q13490

Additional cIAP-1/HIAP-2 Products

Product Documents for cIAP-1/HIAP-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for cIAP-1/HIAP-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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