Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1020221
View all CRISPR-Cas13a Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Leptotrichia wadei Cas13a
Lys2-Glu1152
Accession # WP_021746774

Specificity

Detects CRISPR-Cas13a in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CRISPR-Cas13a Antibody

Detection of CRISPR-Cas13a by Western Blot.

Western blot shows recombinant Leptotrichia wadei Cas 13a protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-CRISPR-Cas13a Monoclonal Antibody (Catalog # MAB10462) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CRISPR-Cas13a at approximately 143 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for CRISPR-Cas13a Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant Leptotrichia wadei Cas13a protein

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRISPR-Cas13a

Long Name

CRISPR-associated Endoribonuclease 13a

UniProt

WP_021746774

Additional CRISPR-Cas13a Products

Product Documents for CRISPR-Cas13a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for CRISPR-Cas13a Antibody

For research use only

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