CXCR4 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56437

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Sheep

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CXCR4 Primary Antibodies »

Product Specifications

Immunogen

Rabbit anti-CXCR4 polyclonal antibody was raised against a peptide corresponding to amino acids 328-338 of human CXCR4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CXCR4 Antibody - BSA Free

Knockdown Validated: CXCR4 Antibody [NB100-56437]

Western Blot: CXCR4 Antibody [NB100-56437]

Western Blot: CXCR4 Antibody [NB100-56437] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions.
Western Blot: CXCR4 Antibody [NB100-56437]

Western Blot: CXCR4 Antibody [NB100-56437]

Western Blot: CXCR4 Antibody [NB100-56437] - Detection of 15 ug in HeLa cells with antibody concentration at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-56437]

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-56437]

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-56437] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: CXCR4 Antibody [NB100-56437]

Immunohistochemistry-Paraffin: CXCR4 Antibody [NB100-56437]

Immunohistochemistry-Paraffin: CXCR4 Antibody [NB100-56437] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue).
CXCR4 Antibody - BSA Free

Western Blot: CXCR4 Antibody - BSA Free [NB100-56437] -

CXCL12 secreted from DFs induces AR and CXCR4 in DPCs. The effects of rCXCL12 on the expression of AR and CXCR4 in DPCs were observed using qRT-PCR (A,B) and Western blot analysis (C). rCXCL12 increased the mRNA and protein expression of the AR and CXCR4 in DPCs. $ p < 0.05, $$ p < 0.01 vs. Control. (D) DFs were treated with 100 nM TP or DHT for 48 h and the culture medium (CM) was collected. CM from DFs treated with TP and DHT (DFCMTP and DFCMDHT) significantly reduced hair length in human hair organ culture. $ p < 0.05 vs. DFCM, n = 10. The dollar sign ($) indicates differences in a one-way ANOVA. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/3/1705), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CXCR4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

reported in scientific literature (Nimmagadda et al (2010))

Immunohistochemistry-Paraffin

1:10 - 1:500. Use reported in scientific literature (Zagzag (2005))

Western Blot

1 - 2 ug/mL
Application Notes
In HeLa, a 45 kDa band is observed.

Reviewed Applications

Read 1 review rated 5 using NB100-56437 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCR4

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors1. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (2,3). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (4,5). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (8). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (2). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (2,5,10). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites (10,11).

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat)

Gene Symbol

CXCR4

UniProt

P61073

Additional CXCR4 Products

Product Documents for CXCR4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CXCR4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CXCR4 Antibody - BSA Free

Customer Reviews for CXCR4 Antibody - BSA Free (1)

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  • CXCR4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human MDA-MB-231 cells
    Species: Human
    Verified Customer | Posted 03/07/2020
    CXCR4 Antibody - BSA Free NB100-56437

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Protocols

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FAQs for CXCR4 Antibody - BSA Free

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  • Q: Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.

    A:

    CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

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