E-Cadherin Antibody (7H12)

Novus Biologicals | Catalog # NBP2-19051

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Monkey

Cited:

Human, Mouse

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, COMET

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H12
View all E-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human E-Cadherin expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Epithelial Cell Marker, Adherens Junctions Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for E-Cadherin Antibody (7H12)

E-Cadherin Antibody (7H12) - BSA Free

Detection of E-Cadherin in Human Breast via Multiplex Immunofluorescence staining on COMET™

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Novus Catalog # NBP2-19051) at 1:1000 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
E-Cadherin Antibody (7H12) - BSA Free

Detection of E-Cadherin in Human Liver via Multiplex Immunofluorescence staining on COMET™

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # NBP2-19051) at 1:1000 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
E-Cadherin Antibody (7H12) - BSA Free

Detection of E-Cadherin in Human Colon Cancer via Multiplex Immunofluorescence staining on COMET™

E-Cadherin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # NBP2-19051) at 1:1000 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - Human kidney distal tubules stained with E-CAD antibody. Secondary antibody: Donkey anti-Mouse Alexa Fluor 555. Imaging with a 40x objective from Zeiss Observer microscope with Apotome2. Image from verified customer review.
Western Blot: E-Cadherin Antibody (7H12)BSA Free [NBP2-19051]

Western Blot: E-Cadherin Antibody (7H12)BSA Free [NBP2-19051]

E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0013.jpg
Western Blot: E-Cadherin Antibody (7H12)BSA Free [NBP2-19051]

Western Blot: E-Cadherin Antibody (7H12)BSA Free [NBP2-19051]

Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of E-Cadherin in LNCAP (1), A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - ICC staining of E-Cadherin in human colon cancer sphere. Image from verified customer review.
Western Blot: E-Cadherin Antibody (7H12)BSA Free [NBP2-19051]

Western Blot: E-Cadherin Antibody (7H12)BSA Free [NBP2-19051]

E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0014.jpg
Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - Human colonic tissue was stained with E-Cadherin antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin antibody in mouse colon cancer tissue. Antibody dilution 1:500. Image from verified customer review.
Flow Cytometry: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Flow Cytometry: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Flow Cytometry: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of Hela cells using E-Cadherin mouse mAb (green) and negative control (purple).
Western Blot: E-Cadherin Antibody (7H12)BSA Free [NBP2-19051]

Western Blot: E-Cadherin Antibody (7H12)BSA Free [NBP2-19051]

E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0015.jpg
Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051]

Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin in gastric cancer tissues (left) and lung cancer tissues (right) with DAB staining.
E-Cadherin Antibody (7H12) - BSA Free

Western Blot: E-Cadherin Antibody (7H12) - BSA Free [NBP2-19051] -

Mitochondrial fission regulates migration & invasion in BRAFV600E CRC cells through glucose metabolic reprograming. (A) Relative cell invasion post vemurafenib treatment in BRAFV600E cells (n=3, mean ± SEM); statistical analysis was done by unpaired t-test followed by Welch’s correction assuming unequal variance in means, corresponding p values shown. (B) Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAFV600E cells. Relative cellular invasion post (C) DRP1 silencing, (D) PDK1 silencing, & (E) 2-deoxyglucose treatment in Colo205 & HT29 cells (n=3, mean± SEM); statistical analysis was done by unpaired t-test followed by Welch’s correction assuming unequal variance in means, corresponding p values shown. (F) Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon DRP1 silencing, PDK1 knockdown, & 2-DG treatment in Colo205 & HT29 cells. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for E-Cadherin Antibody (7H12)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Multiplex Immunofluorescence

1:1000

Western Blot

1:500-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 20605541).

Reviewed Applications

Read 7 reviews rated 4.4 using NBP2-19051 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-Cadherin is a transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-Cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Tissue specificity: Non-neural epithelial tissues.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human)

Gene Symbol

CDH1

UniProt

P12830

Additional E-Cadherin Products

Product Documents for E-Cadherin Antibody (7H12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for E-Cadherin Antibody (7H12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for E-Cadherin Antibody (7H12)

Customer Reviews for E-Cadherin Antibody (7H12) (7)

4.4 out of 5
7 Customer Ratings
5 Stars
57%
4 Stars
29%
3 Stars
14%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 7 reviews Showing All
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  • E-Cadherin Antibody (7H12)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Colon cancer tissue
    Species: Mouse
    Verified Customer | Posted 02/01/2022
    Mouse colon cancer tissue
    Antibody dilution 1:500
    E-Cadherin Antibody (7H12) NBP2-19051
  • E-Cadherin Antibody (7H12)
    Name: David Ivancic
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human kidney
    Species: Human
    Verified Customer | Posted 08/08/2018
    Human Kidney Distal Tubules Stained with ECAD Antibody
    Taken with a 40x Objective from Zeiss Observer microscope with Apotome2 Secondary antibody: Donkey Anti Mouse 555
    E-Cadherin Antibody (7H12) NBP2-19051
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human breast cancer cell lines
    Species: Human
    Verified Customer | Posted 09/02/2015
  • Name: Arun Satelli
    Application: Immunohistochemistry
    Sample Tested: Human colonic tissue
    Species: Human
    Verified Customer | Posted 11/04/2014
    e-Cadherin IHC
    E-Cadherin Antibody (7H12) NBP2-19051
  • Name: Arun Satelli
    Application: Flow Cytometry
    Sample Tested: Human colon cancer cell line HT29
    Species: Human
    Verified Customer | Posted 11/04/2014
    E-Cadherin Flow
    E-Cadherin Antibody (7H12) NBP2-19051
  • Name: Arun Satelli
    Application: Immunocytochemistry
    Sample Tested: Human colon cancer sphere
    Species: Human
    Verified Customer | Posted 10/08/2014
    E-Cadherin
    E-Cadherin Antibody (7H12) NBP2-19051
  • Name: Bryan Tinsley
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/13/2014
    Western blot analysis of extracts from MCF7 cells using E-Cadherin antibody (NBP2-19051, 1:100).
    E-Cadherin Antibody (7H12) NBP2-19051

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Showing  1 - 5 of 7 reviews Showing All

Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail