Endoglin/CD105 Antibody (3A9) - BSA Free
Novus Biologicals | Catalog # NBP2-22122
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
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Label
Antibody Source
Format
Product Specifications
Immunogen
Reactivity Notes
Marker
Clonality
Host
Isotype
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Endoglin/CD105 Antibody (3A9) - BSA Free
![Western Blot: Endoglin/CD105 Antibody (3A9)BSA Free [NBP2-22122]](https://resources.rndsystems.com/images/products/Endoglin-CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0006.jpg "Western Blot: Endoglin/CD105 Antibody (3A9)BSA Free [NBP2-22122]")
Western Blot: Endoglin/CD105 Antibody (3A9)BSA Free [NBP2-22122]
Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.![Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]](https://resources.rndsystems.com/images/products/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0014.jpg "Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]")
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]
Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Human endometrial stromal cells stained with Endoglin/CD105 antibody. Image from verified customer review.![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]](https://resources.rndsystems.com/images/products/Endoglin-CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0013.jpg "Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]")
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Immunohistochemical staining of endoglin (CD105) in B16F10 murine melanoma tumor model. IHC-P image submitted by a verifed customer review.![Western Blot: Endoglin/CD105 Antibody (3A9)BSA Free [NBP2-22122]](https://resources.rndsystems.com/images/products/CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0002.jpg "Western Blot: Endoglin/CD105 Antibody (3A9)BSA Free [NBP2-22122]")
Western Blot: Endoglin/CD105 Antibody (3A9)BSA Free [NBP2-22122]
Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates.![Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]](https://resources.rndsystems.com/images/products/CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]")
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]
Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red).![Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]](https://resources.rndsystems.com/images/products/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-NBP2-22122-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]")
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]
Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. ICC/IF image submitted by a verified customer review.![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]](https://resources.rndsystems.com/images/products/CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0005.jpg "Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]")
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.![ELISA: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]](https://resources.rndsystems.com/images/products/CD105-Antibody-3A9-ELISA-NBP2-22122-img0001.jpg "ELISA: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]")
ELISA: Endoglin/CD105 Antibody (3A9) - BSA Free [NBP2-22122]
ELISA: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng).Applications for Endoglin/CD105 Antibody (3A9) - BSA Free
ELISA
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry-Paraffin
Western Blot
Reviewed Applications
Read 3 reviews rated 5 using NBP2-22122 in the following applications:
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Background: Endoglin/CD105
Additional Endoglin/CD105 Products
Product Documents for Endoglin/CD105 Antibody (3A9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Endoglin/CD105 Antibody (3A9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Endoglin/CD105 Antibody (3A9) - BSA Free
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Customer Reviews for Endoglin/CD105 Antibody (3A9) - BSA Free (3)
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Customer Images
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Application: ImmunocytochemistrySample Tested: Human endometrial stromal cellsSpecies: HumanVerified Customer | Posted 02/17/2022Human endometrial stromal cells
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Application: Immunohistochemistry-ParaffinSample Tested: B16-F10 mouse melanoma cell lineSpecies: MouseVerified Customer | Posted 08/03/2021Immunohistochemical staining of endoglin (CD105) in B16F10 murine melanoma tumor model.
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Application: ImmunocytochemistrySample Tested: HMSC cellsSpecies: HumanVerified Customer | Posted 01/07/2016CD105 expression in HMSC cells
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Endoglin/CD105 Antibody (3A9) - BSA Free
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Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
-
Q: I would like to know the concentration and purity of this antibody, and whether you could provide a purified format of this anti-CD105.
A: This product is provided as ammonium sulfate precipitated ascites. The concentration of this preparation is 1.0mg/ml (total IgG). Unfortunately, this product is not available in any other format at this time as the clone is licensed from a collaborator.
-
Q: Regarding the anti-endoglin antiboy clone 3A9, what antigen sequence was it raised against?
A: Our product NBP2-22122 was raised against an immunogen that falls between amino acids 330-600 of the human Endoglin protein
-
Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
A:
We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
-
Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
-
Q: I would like to know the concentration and purity of this antibody, and whether you could provide a purified format of this anti-CD105.
A: This product is provided as ammonium sulfate precipitated ascites. The concentration of this preparation is 1.0mg/ml (total IgG). Unfortunately, this product is not available in any other format at this time as the clone is licensed from a collaborator.
-
Q: Regarding the anti-endoglin antiboy clone 3A9, what antigen sequence was it raised against?
A: Our product NBP2-22122 was raised against an immunogen that falls between amino acids 330-600 of the human Endoglin protein
-
Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
A:
We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
-
Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
-
Q: I would like to know the concentration and purity of this antibody, and whether you could provide a purified format of this anti-CD105.
A: This product is provided as ammonium sulfate precipitated ascites. The concentration of this preparation is 1.0mg/ml (total IgG). Unfortunately, this product is not available in any other format at this time as the clone is licensed from a collaborator.
-
Q: Regarding the anti-endoglin antiboy clone 3A9, what antigen sequence was it raised against?
A: Our product NBP2-22122 was raised against an immunogen that falls between amino acids 330-600 of the human Endoglin protein
-
Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
A:
We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
-
Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
-
Q: I would like to know the concentration and purity of this antibody, and whether you could provide a purified format of this anti-CD105.
A: This product is provided as ammonium sulfate precipitated ascites. The concentration of this preparation is 1.0mg/ml (total IgG). Unfortunately, this product is not available in any other format at this time as the clone is licensed from a collaborator.
-
Q: Regarding the anti-endoglin antiboy clone 3A9, what antigen sequence was it raised against?
A: Our product NBP2-22122 was raised against an immunogen that falls between amino acids 330-600 of the human Endoglin protein
-
Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
A:
We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
