Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80719

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A9

Format

Azide and BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human CD105 expressed in E. coli. [Uniprot: P17813]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27322553).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Western Blot: Endoglin/CD105 Antibody (3A9)Azide and BSA Free [NBP2-80719]

Western Blot: Endoglin/CD105 Antibody (3A9)Azide and BSA Free [NBP2-80719]

Western Blot: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - HMSC cells stained with anti-CD105 antibody. Image from verified customer review. Image from the standard format of this antibody.
Immunohistochemistry: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunohistochemistry: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunohistochemistry: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining. Image from the standard format of this antibody.
Western Blot: Endoglin/CD105 Antibody (3A9)Azide and BSA Free [NBP2-80719]

Western Blot: Endoglin/CD105 Antibody (3A9)Azide and BSA Free [NBP2-80719]

Western Blot: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red). Image from the standard format of this antibody.
ELISA: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

ELISA: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

ELISA: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.

Applications for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000. Use reported by customer review

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endoglin/CD105

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia.

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All
  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

  • Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?

    A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

  • Q: We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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