Equine IFN-gamma Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1586
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Equine IFN-gamma Biotinylated Antibody Summary

Species Reactivity
Equine
Specificity
Detects equine IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant canine IFN‑ gamma is observed, less than 6% cross-reactivity with recombinant bovine IFN-gamma and recombinant feline IFN-gamma is observed, and less than 0.2% cross‑reactivity with recombinant human IFN-gamma, recombinant mouse IFN-gamma, recombinant rat IFN-gamma, recombinant porcine IFN‑ gamma, recombinant rhesus macaque IFN-gamma, and recombinant cotton rat IFN-gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant equine IFN-gamma
Ala25-Gln166
Accession # P42160
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Equine IFN-gamma (Catalog # 1586-HG)

Equine IFN-gamma Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Equine IFN‑ gamma Antibody (Catalog # AF1586)

Standard: Recombinant Equine IFN-gamma Protein (Catalog # 1586-HG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature equine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3, 4). It shares 73%‑82% amino acid sequence identity with bovine, canine, feline, and porcine IFN-gamma and 42%‑64% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Grunig, G. et al. (1994) Immunogenetics 39:448.
  4. Curran, J.A. et al. (1994) DNA Seq. 4:405.
  5. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  6. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  7. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  8. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  9. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  10. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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