FITC Antibody

R&D Systems | Catalog # G-148-C

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Mouse

Applications

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FITC Primary Antibodies »

Product Specifications

Immunogen

FITC-KLH (Fluorescein-isothiocyanate-coupled Keyhole Limpet Hemocyanin)

Specificity

Detects Fluorescein Isothiocyanate (FITC) in direct ELISAs. In direct ELISAs, nocross-reactivity with Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 647,Alexa Fluor® 700, Alexa Fluor® 750, NorthernLights™ NL493, NorthernLights™ 557,and NorthernLights™ 637 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL

Formulation

Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and Sodium Azide with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FITC

Fluorescein isothiocyante (FITC) is a derivative of fluorescein modified with an isothiocyanate reactive group (-N=C=S).  FITC is often used to label antibodies for immunofluorescent cytochemistry and histochemistry. FITC can be also conjugated to proteins and RNA and DNA probes because such conjugation does not impair biological activity of these molecules. In some experimental setups FITC fluorescence may need to be enhanced, which is done by employing anti-FITC antibodies conjugated either to FITC or other fluorophores with similar excitation and emission spectra.

Long Name

Fluorescein Isothiocyanate

Alternate Names

Fluorescein Isothiocyanate

Additional FITC Products

Product Documents for FITC Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for FITC Antibody

For research use only

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FAQs

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