GATA-3 Antibody (CL8660) - BSA Free

Novus Biologicals | Catalog # NBP2-88917

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL8660

Format

BSA Free
View all GATA-3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P23771, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GATA-3 Antibody (CL8660) - BSA Free

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented for the same tissues.
Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917]

Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917]

Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human cell line TD47D.
Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunocytochemistry/Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of MCF7 cells using the Anti-GATA3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human kidney shows strong nuclear positivity in a small subset of cells in glomeruli.
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917]

Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.
GATA-3 Antibody (CL8660) - BSA Free Chromatin Immunoprecipitation-exo-Seq: GATA-3 Antibody (CL8660) - BSA Free [NBP2-88917]

Chromatin Immunoprecipitation-exo-Seq: GATA-3 Antibody (CL8660) - BSA Free [NBP2-88917]

ChIP-Exo-Seq composite graph for Anti-GATA3 (NBP2-88917) tested in MCF7 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for GATA-3 Antibody (CL8660) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATA-3

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Alternate Names

GATA3

Gene Symbol

GATA3

Additional GATA-3 Products

Product Documents for GATA-3 Antibody (CL8660) - BSA Free

Certificate of Analysis

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Product Specific Notices for GATA-3 Antibody (CL8660) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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