Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glut2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human GLUT2 protein (between residues 50-150) [UniProt# P11168]

Localization

Integral membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Glut2 Antibody - BSA Free (NBP2-22218) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Glut2 Antibody: Cited in 14 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Glut2 Antibody - BSA Free

Western Blot: Glut2 AntibodyBSA Free [NBP2-22218]

Western Blot: Glut2 AntibodyBSA Free [NBP2-22218]

Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution. Image was submitted via customer review.
Immunocytochemistry/ Immunofluorescence: Glut2 Antibody - BSA Free [NBP2-22218]

Immunocytochemistry/ Immunofluorescence: Glut2 Antibody - BSA Free [NBP2-22218]

Immunocytochemistry/Immunofluorescence: Glut2 Antibody [NBP2-22218] - Glut2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: Glut2 Antibody - BSA Free [NBP2-22218]

Immunohistochemistry-Paraffin: Glut2 Antibody - BSA Free [NBP2-22218]

Glut2-Antibody-Immunohistochemistry-Paraffin-NBP2-22218-img0009.jpg
Western Blot: Glut2 AntibodyBSA Free [NBP2-22218]

Western Blot: Glut2 AntibodyBSA Free [NBP2-22218]

Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glucose Transporter GLUT2 in human pancreas.
Immunohistochemistry: Glut2 Antibody - BSA Free [NBP2-22218]

Immunohistochemistry: Glut2 Antibody - BSA Free [NBP2-22218]

Immunohistochemistry: Glut2 Antibody [NBP2-22218] - Staining of Glucose Transporter GLUT2 in mouse liver.
Immunohistochemistry-Paraffin: Glut2 Antibody - BSA Free [NBP2-22218]

Immunohistochemistry-Paraffin: Glut2 Antibody - BSA Free [NBP2-22218]

Glut2-Antibody-Immunohistochemistry-Paraffin-NBP2-22218-img0010.jpg

Applications for Glut2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1.0 - 2.0 ug/ml
Application Notes
This Glucose Transporter GLUT2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 55 kDa. In ICC/IF membrane staining was observed in HepG2 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 3 reviews rated 4.3 using NBP2-22218 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glut2

SLC2A2 (Solute Carrier Family 2), also known as Glucose Transporter GLUT2, is an integral plasma membrane glycoprotein found in the liver, islet beta cells, intestine, and kidney epithelium. This isoform of the glucose transporter mediates the two way transfer of glucose across the plasma membrane and is responsible for the uptake of glucose in beta cells. Mutations in SLC2A2 lead to Fanconi-Bickel syndrome (FBS), which results in hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Recent studies have shown that mutations in SLC2A2 can cause neonatal diabetes, and therefore may contribute to human insulin secretion (PMID: 22660720). Novel SLC2A2 mutations have also been discovered and are being investigated to determine their roles in FBS as well (PMID: 22145468).

Long Name

Glucose Transporter Type 2

Alternate Names

SLC2A2

Entrez Gene IDs

6514 (Human); 20526 (Mouse)

Gene Symbol

SLC2A2

UniProt

P14246, P11168

Additional Glut2 Products

Product Documents for Glut2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Glut2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Glucose Homeostasis
Metabolism

Citations for Glut2 Antibody - BSA Free

Customer Reviews for Glut2 Antibody - BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Glut2 Antibody - BSA Free
    Name: Zeyuan Zhang
    Application: Western Blot
    Sample Tested: mouse hepatocytes and HepG2 cells
    Species: Mouse
    Verified Customer | Posted 07/31/2023
    mouse primary hepatocyte lysate
    Glut2 Antibody - BSA Free NBP2-22218
  • Glut2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cultured cancer cell lysates
    Species: Human
    Verified Customer | Posted 01/13/2020
    GLUT2 in human cancer cell
    Glut2 Antibody - BSA Free NBP2-22218
  • Glut2 Antibody
    Name: Kohsuke Kataoka
    Application: Western Blot
    Sample Tested: Min6 mouse insulinoma whole cell lysate
    Species: Mouse
    Verified Customer | Posted 02/07/2018
    Western blot analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution.
    Glut2 Antibody - BSA Free NBP2-22218

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Protocols

View specific protocols for Glut2 Antibody - BSA Free (NBP2-22218):


Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.



Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 25 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute anti-Glucose Transporter GLUT2 primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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