Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Common beta Chain Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Common beta Chain
Gly23-Trp443
Accession # P32927

Specificity

Detects recombinant human (rh) Common beta  Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GM-CSF R alpha, rhG-CSF R and rhM-CSF R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Common  beta  Chain Antibody

Detection of Common beta Chain antibody in Human PBL antibody by Flow Cytometry.

Detection of Common beta Chain in Human PBL by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Goat Anti-Human Common beta Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF906, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109).

Applications for Human Common  beta  Chain Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood lymphocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human Common  beta  Chain

Reviewed Applications

Read 1 review rated 3 using AF906 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Common beta Chain

The common beta chain, also known as CD131, is a transmembrane protein that associates with the ligand-specific IL-3 R alpha, IL-5 R alpha, or GM-CSF R alpha proteins to form high affinity receptor complexes. beta c is expressed on a variety of hematopoietic cells.

Alternate Names

CD131, CSF2RB, GM-CSF R beta, IL-3 R beta, IL-5 R beta

Entrez Gene IDs

1439 (Human); 12983 (Mouse); 102143353 (Cynomolgus Monkey)

Gene Symbol

CSF2RB

UniProt

P32927

Additional Common beta Chain Products

Product Documents for Human Common  beta  Chain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Common  beta  Chain Antibody

For research use only

Citations for Human Common  beta  Chain Antibody

Customer Reviews for Human Common  beta  Chain Antibody (1)

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Customer Images

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  • Human Common  beta  Chain Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 04/11/2022
    Human Common  beta  Chain Antibody AF906

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