The common beta chain, also known as CD131, is a transmembrane protein that associates with the ligand-specific IL-3 R alpha, IL-5 R alpha, or GM-CSF R alpha proteins to form high affinity receptor complexes. beta c is expressed on a variety of hematopoietic cells.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Flow Cytometry, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Common beta Chain
Gly23-Trp443
Accession # P32927
Gly23-Trp443
Accession # P32927
Specificity
Detects recombinant human (rh) Common beta Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GM-CSF R alpha, rhG-CSF R and rhM-CSF R is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Common beta Chain Antibody
Detection of Common beta Chain in Human PBL by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Goat Anti-Human Common beta Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF906, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109).Applications for Human Common beta Chain Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human Common beta Chain
Sample: Recombinant Human Common beta Chain
Reviewed Applications
Read 1 review rated 3 using AF906 in the following applications:
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Common beta Chain
Alternate Names
CD131, CSF2RB, GM-CSF R beta, IL-3 R beta, IL-5 R beta
Gene Symbol
CSF2RB
UniProt
Additional Common beta Chain Products
Product Documents for Human Common beta Chain Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Common beta Chain Antibody
For research use only
Citations for Human Common beta Chain Antibody
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1 Customer Rating
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Melanoma tissueSpecies: HumanVerified Customer | Posted 04/11/2022
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Associated Pathways
