Human Common  beta  Chain Antibody

Catalog # Availability Size / Price Qty
AF906
AF906-SP
Detection of Common  beta  Chain in Human PBL by Flow Cytometry.
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Product Details
Citations (2)
FAQs
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Reviews (1)

Human Common  beta  Chain Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human (rh) Common beta  Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GM-CSF R alpha, rhG-CSF R and rhM-CSF R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Common beta Chain
Gly23-Trp443
Accession # P32927
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Common  beta  Chain
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Common  beta  Chain antibody in Human PBL antibody by Flow Cytometry. View Larger

Detection of Common beta Chain in Human PBL by Flow Cytometry. Human peripheral blood lymphocytes were stained with Goat Anti-Human Common beta Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF906, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common beta Chain

The common beta chain, also known as CD131, is a transmembrane protein that associates with the ligand-specific IL-3 R alpha, IL-5 R alpha, or GM-CSF R alpha proteins to form high affinity receptor complexes. beta c is expressed on a variety of hematopoietic cells.

Entrez Gene IDs
1439 (Human); 12983 (Mouse); 102143353 (Cynomolgus Monkey)
Alternate Names
beta, low-affinity; CD131 antigen; CD131; CDw131; colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage); Common beta Chain; CSF2RB; cytokine receptor common subunit beta; GM-CSF R beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; GM-CSF/IL-3/IL-5 receptor common beta-chain; IL-3 R beta; IL3RB; IL-5 R beta; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity)

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Citations for Human Common  beta  Chain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Three lysine residues in the common beta chain of the interleukin-5 receptor are required for Janus kinase (JAK)-dependent receptor ubiquitination, endocytosis, and signaling.
    Authors: Lei JT, Mazumdar T, Martinez-Moczygemba M
    J. Biol. Chem., 2011-09-30;286(46):40091-103.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Separate endocytic pathways regulate IL-5 receptor internalization and signaling.
    Authors: Lei JT, Martinez-Moczygemba M
    J. Leukoc. Biol., 2008-05-29;84(2):499-509.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human Common  beta  Chain Antibody

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Human Common  beta  Chain Antibody
By Anonymous on 04/11/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human