Human/Mouse/Rat TC-PTP Antibody Summary
Accession # P17706
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat TC-PTP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TC-PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TC-PTP at approximately 45 to 48 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Detection of Human TC‑PTP by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for TC-PTP at approximately 51 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat TC-PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kDa isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kDa isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).
- Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
- Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
- Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
- Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
- Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
- Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
- You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
- Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
- Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.
Citation for Human/Mouse/Rat TC-PTP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase.
Authors: Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A
J. Biol. Chem., 2010;285(51):40114-24.
Sample Types: Cell Lysates
Applications: Western Blot
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