Human/Mouse/Rat TC-PTP Antibody

Catalog # Availability Size / Price Qty
AF1930
AF1930-SP
Human/Mouse/Rat TC-PTP Antibody in Data
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Product Details
Citations (1)
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Human/Mouse/Rat TC-PTP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat TC-PTP in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TC-PTP
Pro2-Asn314
Accession # P17706
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat TC-PTP antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat TC-PTP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TC-PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TC-PTP at approximately 45 to 48 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western Detection of Human TC‑PTP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human TC‑PTP by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for TC-PTP at approximately 51 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat TC-PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TC-PTP

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kDa isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kDa isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).

References
  1. Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
  2. Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
  3. Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
  4. Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
  5. Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
  6. Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
  7. You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
  8. Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
  9. Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.
Long Name
T Cell Protein Tyrosine Phosphatase
Entrez Gene IDs
5771 (Human); 19255 (Mouse)
Alternate Names
protein tyrosine phosphatase, non-receptor type 2; PTP2; PTPN2; PTPTPTN2; T-cell protein tyrosine phosphatase; T-cell protein-tyrosine phosphatase; TCELLPTP; TCPTP; TC-PTP; TCPTPEC 3.1.3.48; tyrosine-protein phosphatase non-receptor type 2

Product Datasheets

Citation for Human/Mouse/Rat TC-PTP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase.
    Authors: Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A
    J. Biol. Chem., 2010;285(51):40114-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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