His Tag HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
MAB050H
MAB050H-SP
Detection  of  His Tag by Western Blot.
2 Images
Product Details
Citations (4)
FAQs
Reviews (3)

His Tag HRP-conjugated Antibody Summary

Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags at the amino- or carboxyl‑terminus.
Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
His-tagged peptide
Formulation
Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
HRP (Horseradish Peroxidase)

Applications

Recommended Concentration
Sample
Western Blot
1:4000 dilution
See below
Simple Western
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of  His Tag by Western Blot. View Larger

Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with a 1:4000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). Specific bands were detected for His Tag at approximately 32 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of His Tag by Simple WesternTM. View Larger

Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1, loaded at 0.2 mg/mL. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated) using a 1:1000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
  • 6 months, 2 to 8 °C under sterile conditions after opening.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine

Product Datasheets

Citations for His Tag HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide
    Authors: R Biedro?, A Peru?, S Józefowski
    PLoS ONE, 2016;11(4):e0153558.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  2. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.
    Authors: Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S
    PLoS ONE, 2015;10(4):e0123293.
    Species: Mouse
    Sample Types: Protein
    Applications: ELISA detection
  3. Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro.
    Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L
    Chem. Biol., 2006;13(5):549-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  4. Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1.
    Authors: Wohl A, Troilo H, Collins R, Baldock C, Sengle G
    J Biol Chem, 0;291(24):12732-46.
    Species: Human
    Sample Types: Protein

FAQs

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Reviews for His Tag HRP-conjugated Antibody

Average Rating: 5 (Based on 3 Reviews)

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His Tag HRP-conjugated Antibody
By Anonymous on 07/13/2018
Application: Excellent Antibody. Detects 6xhis positive control, almost instantaneously. Sample Tested: Protein Lysate,6xhis positive control Species: Bacterial

TMB Detected Western Blot


His Tag HRP-conjugated Antibody
By Anonymous on 06/20/2018
Application: Simple Western Sample Tested: Purified protein Species: Human

His Tag HRP-conjugated Antibody
By Anonymous on 05/17/2017
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Mouse