Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Bacteria, Bacteria - Vibrio parahaemolyticus, E. coli, Parasite - Fasciola hepatica (liver fluke), Yeast

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Neutralization, Flow Cytometry, Binding Assay, Bioassay, ELISA Detection, ELISA Development, ELISA Development (Detection), Functional Assay

Label

HRP

Antibody Source

Monoclonal Mouse IgG1 Clone # AD1.1.10
View all His Tag Primary Antibodies »

Product Specifications

Immunogen

His-tagged peptide

Specificity

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags at the amino- or carboxyl‑terminus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag Horseradish Peroxidase-conjugated Antibody

Detection of His Tag antibody by Western Blot.

Detection of His Tag by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with a 1:4000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). Specific bands were detected for His Tag at approximately 32 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of His Tag antibody by Simple WesternTM.

Detection of His Tag by Simple WesternTM.

Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1, loaded at 0.2 mg/mL. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated) using a 1:1000 dilution of Mouse Anti-His Tag HRP-conjugated Monoclonal Antibody (Catalog # MAB050H). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for His Tag Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

Simple Western

1:1000 dilution
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins

Western Blot

1:4000 dilution
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins

Reviewed Applications

Read 6 reviews rated 5 using MAB050H in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack (-SP) is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for His Tag Horseradish Peroxidase-conjugated Antibody

For research use only

Citations for His Tag Horseradish Peroxidase-conjugated Antibody

Customer Reviews for His Tag Horseradish Peroxidase-conjugated Antibody (6)

5 out of 5
6 Customer Ratings
5 Stars
100%
4 Stars
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3 Stars
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2 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 6 reviews Showing All
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  • His Tag Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: E. coli
    Verified Customer | Posted 11/13/2023
    This antibody works very well to detect his-tagged antigens via Western blot. In the blot shown here, the His antibody was used to detect recombinant TcfA, TcfB, and TcfC. The bands are clear with little to no background. Because this is HRP labeled, there is no need for a secondary antibody, which is helpful to speed up the assay. I will use a secondary to increase band intensity.
    His Tag Horseradish Peroxidase-conjugated Antibody MAB050H
  • His Tag Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: HEK293 human embryonic kidney cell line and SERPIN
    Species: Mouse
    Verified Customer | Posted 02/21/2023
    His Tag Horseradish Peroxidase-conjugated Antibody MAB050H
  • His Tag Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Extracts
    Species: Bacterial
    Verified Customer | Posted 11/26/2019
    Western blot was made in 5% BSA, antibody worked beautifully at 1:1000 dilution.
    His Tag Horseradish Peroxidase-conjugated Antibody MAB050H
  • His Tag HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Protein Lysate and 6xhis positive control
    Species: Bacterial
    Verified Customer | Posted 07/13/2018
    TMB Detected Western Blot
    Excellent Antibody. Detects 6xhis positive control, almost instantaneously.
    His Tag Horseradish Peroxidase-conjugated Antibody MAB050H
  • His Tag HRP-conjugated Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 06/20/2018
    His Tag Horseradish Peroxidase-conjugated Antibody MAB050H
  • His Tag HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Mouse
    Verified Customer | Posted 05/17/2017
    His Tag Horseradish Peroxidase-conjugated Antibody MAB050H

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FAQs for His Tag Horseradish Peroxidase-conjugated Antibody

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  • Q: Can the His Tag Antibody (MAB050) recognize all His-tagged proteins?

    A: MAB050 has been validated specifically for the detection of His-tags localized to the amino- or carboxyl-terminus of a peptide.  It is also expected to detect any series of 6 or more histidine residues as long as this epitope is conformationally accessible. For applications utilizing linearized proteins, it is likely that MAB050 will have access to any consecutive histidine regions.  If used with protein in its native conformation, there is the potential that the protein will be folded such that the antibody is not able to bind its target.

  • Q: What is the molecular weight of an HRP-conjugated antibody?

    A: The average ratio of HRP:antibody is estimated to be 2 to 4 HRP molecules per antibody molecule based on A403 and A280 readings of the final conjugate. Using ~44kDa as the molecular weight of HRP and 150kDa as the molecular weight of IgG, the theoretical average MW of the conjugated antibody is 238kDa to 326kDa. The HRP-conjugation does vary quite a bit with each antibody, but does not vary as much with each conjugation of the same antibody, especially if it is a monoclonal.

  • Q: Can the His Tag Antibody (MAB050) recognize all His-tagged proteins?

    A: MAB050 has been validated specifically for the detection of His-tags localized to the amino- or carboxyl-terminus of a peptide.  It is also expected to detect any series of 6 or more histidine residues as long as this epitope is conformationally accessible. For applications utilizing linearized proteins, it is likely that MAB050 will have access to any consecutive histidine regions.  If used with protein in its native conformation, there is the potential that the protein will be folded such that the antibody is not able to bind its target.

  • Q: What is the molecular weight of an HRP-conjugated antibody?

    A: The average ratio of HRP:antibody is estimated to be 2 to 4 HRP molecules per antibody molecule based on A403 and A280 readings of the final conjugate. Using ~44kDa as the molecular weight of HRP and 150kDa as the molecular weight of IgG, the theoretical average MW of the conjugated antibody is 238kDa to 326kDa. The HRP-conjugation does vary quite a bit with each antibody, but does not vary as much with each conjugation of the same antibody, especially if it is a monoclonal.

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