hnRNP A1 Antibody (4B10) - BSA Free

Novus Biologicals | Catalog # NB100-672

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray, Knockdown Validated

Cited:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4B10

Format

BSA Free
View all hnRNP A1 Primary Antibodies »

Product Specifications

Immunogen

Full length partially purified human hnRNP A1. [UniProt# P09651]

Reactivity Notes

Not yet tested in other species.

Localization

Nucleus. Cytoplasm. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes.

Specificity

Does not react with hnRNP A2 or B

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for hnRNP A1 Antibody (4B10) - BSA Free

Knockdown Validated: hnRNP A1 Antibody (4B10) - BSA Free [NB100-672]

Western Blot: hnRNP A1 Antibody (4B10) - BSA Free [NB100-672]

Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and hnRNP A1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human hnRNP A1 Monoclonal Antibody (Catalog # NB100-672) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for hnRNP A1 at approximately 35 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: hnRNP A1 Antibody (4B10)BSA Free [NB100-672]

Western Blot: hnRNP A1 Antibody (4B10)BSA Free [NB100-672]

Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Whole cell protein from HeLa (lane 1), A431 (lane 2), Hek293 (lane 3) and MCF7 (lane 4) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-hnRNP in 1% milk. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). Both antibodies were detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: hnRNP A1 Antibody (4B10) - BSA Free [NB100-672]

Immunocytochemistry/ Immunofluorescence: hnRNP A1 Antibody (4B10) - BSA Free [NB100-672]

Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody (4B10) [NB100-672] - The hnRNP A1 (4B10) antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Western Blot: hnRNP A1 Antibody (4B10)BSA Free [NB100-672]

Western Blot: hnRNP A1 Antibody (4B10)BSA Free [NB100-672]

Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-hnRNP-A1 Clone: 4B10 (NB100-672). Lanes: 1-HeLa, 2-Jurkat, 3-HEK-293T, 4-WiDr, 5-NCI-H1299, 6-F9.
hnRNP A1 Antibody (4B10) - BSA Free

Western Blot Shows Human hnRNP A1 Specificity Using Knockout Cell Line.

Western blot shows lysates of HEK 293T cell line and hnRNP A1 knockout HEK 293T cell line (KO). Nitrocellulose membrane was probed with hnRNP A1 Antibody (Catalog # NB100-672) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for hnRNP A1 (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. The Ponceau stained transfer of the blot is shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
hnRNP A1 Antibody (4B10) - BSA Free

Immunocytochemistry/ Immunofluorescence of hnRNP A1 Antibody in HEK 293T and hnRNP A1 Knockout HEK 293T Cells in Immunocytochemistry/Immunofluorescence.

HEK 293T WT and hnRNP A1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with hnRNP A1 antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for hnRNP A1 Antibody (4B10) - BSA Free

Application
Recommended Usage

ELISA

Indirect

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

reported in scientific literature (PMID 3129338)

Immunoprecipitation

1:10-1:500

Western Blot

0.25 - 0.5 ug/ml
Application Notes
In WB a band can be seen between 32-35 kDa. This antibody immunoprecipitates the hnRNP complex.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hnRNP A1

RNA polymerase II trans cripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, premRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts.2, 4, 5 Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa.2, 4, 5 hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein binds to its RNA target through a consensus RNA-binding site UAGGGU. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP -A1 from transportin. hnRNP-A1 is ubiquitously expressed with a higher expression in proliferating and/or transformed cells than in differentiated tissues.6

Alternate Names

Helix-destabilizing protein, heterogeneous nuclear ribonucleoprotein A1, heterogeneous nuclear ribonucleoprotein A1B protein, heterogeneous nuclear ribonucleoprotein B2 protein, heterogeneous nuclear ribonucleoprotein core protein A1, hnRNP A1, hnRNP core protein A1, hnRNPA1, hnRNP-A1, HNRPA1MGC102835, nuclear ribonucleoprotein particle A1 protein, single-strand DNA-binding protein UP1, Single-strand RNA-binding protein

Entrez Gene IDs

3178 (Human)

Gene Symbol

HNRNPA1

UniProt

P09651 (Human)

Additional hnRNP A1 Products

Product Documents for hnRNP A1 Antibody (4B10) - BSA Free

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Product Specific Notices for hnRNP A1 Antibody (4B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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