Detects human 4‑1BB/TNFRSF9/CD137 in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse 4-1BB, recombinant human (rh) NGF R, rhTNF RI and rhTNF RII is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human 4-1BB Leu24-Gln186 Accession # Q07011
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Detection of 4‑1BB/TNFRSF9/ CD137 in PHA-treated Human T Cells by Flow Cytometry.
Human T cells were treated for 48 hours with 5 μg/mL PHA then stained with Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF838, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).
4‑1BB/TNFRSF9/CD137 in Human PBMCs.
4‑1BB/TNFRSF9/CD137 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF838) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red ; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 KD glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.
Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.
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