Human 4-1BB/TNFRSF9/CD137 Biotinylated Antibody Summary
Accession # Q07011
Human 4-1BB/TNFRSF9/CD137 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of 4‑1BB/TNFRSF9/
CD137 in PHA-treated Human T Cells by Flow Cytometry. Human T cells were treated for 48 hours with 5 μg/mL PHA then stained with Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF838, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).
4‑1BB/TNFRSF9/CD137 in Human PBMCs. 4‑1BB/TNFRSF9/CD137 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF838) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red ; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 KD glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.
- Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
- Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.
Citation for Human 4-1BB/TNFRSF9/CD137 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Expression of CD137 (4-1BB) on human follicular dendritic cells.
Authors: Lindstedt M, Johansson-Lindbom B, Borrebaeck CA
Scand. J. Immunol., 2003;57(4):305-10.
Sample Types: Whole Cells
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