Human 4-1BB/TNFRSF9/CD137 Antibody
Human 4-1BB/TNFRSF9/CD137 Antibody Summary
Leu24-Gln186
Accession # Q07011
Applications
The ED50 for this effect is typically ≤ 20 μg/mL.
Human 4-1BB/TNFRSF9/CD137 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human 4‑1BB/TNFRSF9/ CD137 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human 4-1BB/TNFRSF9/ CD137-eGFP Fusion. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 4-1BB/ TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody ([catalogNumber:HAF017]]). A specific band was detected for 4-1BB/ TNFRSF9/CD137-eGFP Fusion at approximately 50-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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4‑1BB/TNFRSF9/CD137 in Human Tonsil. 4-1BB/TNFRSF9/CD137 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of 4‑1BB/TNFRSF9/CD137 in PHA-treated Human T Cells by Flow Cytometry. Human T cells were treated for 48 hours with 5 µg/mL PHA then stained with Goat Anti-Human 4-1BB/TNFRSF9/ CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and PE-conjugated anti-human CD3 (Catalog # FAB100P). Quad marker was set based on control antibody (Catalog # AB-108-C) staining.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 4-1BB/TNFRSF9/CD137
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the
TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.
- Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
- Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.
Product Datasheets
Citations for Human 4-1BB/TNFRSF9/CD137 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Anti-CD137 monoclonal antibody enhances trastuzumab-induced, natural killer cell-mediated cytotoxicity against pancreatic cancer cell lines with low human epidermal growth factor-like receptor 2 expression
Authors: T Masu, M Atsukawa, K Nakatsuka, M Shimizu, D Miura, T Arai, H Harimoto, C Kondo, K Kaneko, S Futagami, C Kawamoto, H Takahashi, K Iwakiri
PLoS ONE, 2018;13(12):e0200664.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting peripheral blood pro-inflammatory cytotoxic lymphocytes by inhibiting CD137 expression: novel potential treatment for COPD.
Authors: Hodge G, Holmes M, Jersmann H, Reynolds P, Hodge S
BMC Pulm Med, 2014;14(0):85.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy.
Authors: Zhang H, Snyder KM, Suhoski MM, Maus MV, Kapoor V, June CH, Mackall CL
J. Immunol., 2007;179(7):4910-8.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Functional expression of 4-1BB (CD137) in the inflammatory tissue in Crohn's disease.
Authors: Maerten P, Geboes K, De Hertogh G, Shen C, Cadot P, Bullens DM, Van Assche G, Penninckx F, Rutgeerts P, Ceuppens JL
Clin. Immunol., 2004;112(3):239-46.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
FAQs
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Reviews for Human 4-1BB/TNFRSF9/CD137 Antibody
Average Rating: 5 (Based on 1 Review)
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