Human 4-1BB/TNFRSF9/CD137 Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) 4-1BB, recombinant human (rh) NGF R, rhTNF RI and rhTNF RII is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human 4‑1BB/TNFRSF9/CD137
    Leu24-Gln186
    Accession # Q07011
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
  • Agonist Activity
    Measured by its ability to co-stimulate IFN-gamma secretion by human T cells in the presence of anti-CD3.
    The ED50 for this effect is typically 5 - 20 μg/mL.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed human peripheral blood mononuclear cells
    • Human 4-1BB/TNFRSF9/CD137 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human 4‑1BB/TNFRSF9/CD137 Antibody (Catalog # AF838)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antibody (Catalog # BAF838)
  • ELISA Standard
     
    Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF (Catalog # 838-4B)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human 4‑1BB/TNFRSF9/
CD137 by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human 4-1BB/TNFRSF9/
CD137. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 4‑1BB/
TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).
A specific band was detected for 4‑1BB/
TNFRSF9/CD137 at approximately
50-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of 4‑1BB/TNFRSF9/CD137 in PHA-treated Human T Cells by Flow Cytometry. Human T cells were treated for 48 hours with 5 μg/mL PHA then stained with Goat Anti-Human 4‑1BB/TNFRSF9/
CD137 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF838) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and PE-conjugated anti-human CD3 (Catalog # FAB100P). Quad marker was set based on control antibody (Catalog # AB‑108‑C) staining.
Immunohistochemistry
4‑1BB/TNFRSF9/CD137 in Human Tonsil. 4‑1BB/TNFRSF9/CD137 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human
4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 4-1BB/TNFRSF9/CD137

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the
TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

  • References:
    1. Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
    2. Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.
  • Entrez Gene IDs:
    3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
  • Alternate Names:
    4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Applications
Sample Type
  1. Targeting peripheral blood pro-inflammatory cytotoxic lymphocytes by inhibiting CD137 expression: novel potential treatment for COPD.
    Authors: Hodge G, Holmes M, Jersmann H, Reynolds P, Hodge S
    BMC Pulm Med, 2014;14(0):85.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  2. 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy.
    Authors: Zhang H, Snyder KM, Suhoski MM, Maus MV, Kapoor V, June CH, Mackall CL
    J. Immunol., 2007;179(7):4910-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  3. Functional expression of 4-1BB (CD137) in the inflammatory tissue in Crohn's disease.
    Authors: Maerten P, Geboes K, De Hertogh G, Shen C, Cadot P, Bullens DM, Van Assche G, Penninckx F, Rutgeerts P, Ceuppens JL
    Clin. Immunol., 2004;112(3):239-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
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Normal Goat IgG Control

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We have 1 review tested in 1 application: Flow Cytometry.

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Summary

ApplicationFlow Cytometry
Sample TestedAdult kidney
SpeciesHuman

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