Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 416608
View all ADAMTS4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ADAMTS4
Phe213-Cys685
Accession # O75173

Specificity

Detects human ADAMTS4 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ADAMTS4 Antibody

Human ADAMTS4 ELISA Standard Curve.

Recombinant Human ADAMTS4 protein was serially diluted 2-fold and captured by Mouse Anti-Human ADAMTS4 Monoclonal Antibody (Catalog # MAB43071) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ADAMTS4 Monoclonal Antibody (Catalog # MAB4307) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human ADAMTS4 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human ADAMTS4 Monoclonal Antibody (Catalog # MAB4307).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human ADAMTS4 DuoSet ELISA Kit (Catalog # DY4307-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAMTS4

ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha -2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4‑6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). ADAMTS4 consists of a signal peptide (aa 1 to 51), a pro domain (aa 52 to 212) and a mature chain (aa 213 to 837) containing the following domains: catalytic (aa 218 to 425), disintegrin (aa 437 to 519), TSP type-1 (aa 520 to 575), Cys-rich (aa 577 to 685) and spacer (aa 686 to 837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.

References

  1. Totorella, M. D. et al. (1999) Science 284:1664.
  2. Porter, S. et al. (2005) Biochem. J. 386:15.
  3. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  4. Tortorella, M. D. et al. (2004) J. Biol. Chem. 279:17554.
  5. Struglics, A. et al. (2006) Osteoarthritis Cartilage. 14:101.
  6. Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
  7. Pratta, M. A. et al. (2003) Arthritis Reum. 48:119.
  8. Glasson, S. S. et al. (2004) Arthritis Reum. 50:2547.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 4

Alternate Names

ADMP-1, Aggrecanase 1

Entrez Gene IDs

9507 (Human); 66015 (Rat)

Gene Symbol

ADAMTS4

UniProt

O75173

Additional ADAMTS4 Products

Product Documents for Human ADAMTS4 Antibody

Certificate of Analysis

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Product Specific Notices for Human ADAMTS4 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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