Human AIM2 Antibody Summary
Accession # O14862
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human AIM2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human AIM2 Monoclonal Antibody (Catalog # MAB9965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for AIM2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Human Interferon-inducible protein AIM2 (also known as absent in melanoma 2 or simply AIM2), encoded by the AIM2 gene, is a 343 amino acids (aa), 39 KDa protein with a N-terminal DAPIN (or pyrin) domain (amino acids 1-87) and a C-terminal HIN-200 domain (amino acids 138-337), which is known to have two oligonucleotide-binding folds. Recent research has shown that AIM2 is part of the inflammasome and is involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating the inflammasome in macrophages. Upon binding to DNA, Aim-2 undergoes oligomerization and associates with PYCARD initiating the recruitment of caspase-1 precursors and processing of IL-1b and IL-18. Over aa 71-180, human Aim2 shares 90% aa sequence identity with macaque Aim2.
Citation for Human AIM2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes
Authors: Y Chen, Q Fujuan, E Chen, B Yu, F Zuo, Y Yuan, X Zhao, C Xiao
Mediators Inflamm, 2020;2020(0):1693730.
Sample Types: Cell Lysates
Applications: Western Blot
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