Human Akt1 (E17K Mutation) Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Akt1

Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Substitution of glutamic acid with lysine at amino acid 17 (E17K) occurs in multiple malignancies and results in constitutive Akt1 activation and cell transformation. Within aa 5‑30, human Akt1 shares 100% aa sequence identity with mouse and rat Akt1.

Product Datasheets

Related Research Areas

• Apoptosis Intracellular Kinases
• Chemokine Receptor Intracellular Signaling
• Common gamma Chain Receptor Family
• Diabetic Peripheral Neuropathy
• GDNF Family
• Growth Hormone/IGF-I Axis
• IL-10 Family
• Interferons
• Intracellular Kinases
• Intracellular Kinases in the Akt Pathway
• Intracellular Signaling Molecules in Angiogenesis
• Kinases and Phosphatases in Endothelial Cells
• Neurofibrillary Tangle Formation
• Neurotrophin/Trk Family
• Notch Pathway
• PDGF Family
• Platelet Signaling
• Search Kinases and Phosphatases
• TNF Superfamily Regulators
• VEGF Family