Key Product Details

Species Reactivity

Validated:

Human

Cited:

Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1006614
View all alpha-2A Adrenergic R/ADRA2A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing Human alpha ‑2A Adrenergic R/ADRA2A

Specificity

Detects human alpha ‑2A Adrenergic R/ADRA2A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human alpha ‑2A Adrenergic R/ADRA2A Antibody

Detection of ADRA2A antibody in HEK293 Human Cell Line Transfected with Human ADRA2A and eGFP antibody by Flow Cytometry.

Detection of ADRA2A in HEK293 Human Cell Line Transfected with Human ADRA2A and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2A or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ADRA2A Monoclonal Antibody (Catalog # MAB10129) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human alpha ‑2A Adrenergic R/ADRA2A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human ADRA2A and eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-2A Adrenergic R/ADRA2A

Alpha-2-adrenergic receptors, including ADRA2A, are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both ADRA2A and ADRA2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The ADRA2A subtype inhibited transmitter release at high stimulation frequencies, whereas the ADRA2C subtype modulated neurotransmission at lower levels of nerve activity.

Long Name

Alpha-2A Adrenergic Receptor

Alternate Names

a-2A AdrenergicR, ADRA2A, alpha2A AdrenergicR, ZNF32

Entrez Gene IDs

150 (Human); 11551 (Mouse); 25083 (Rat)

Gene Symbol

ADRA2A

Additional alpha-2A Adrenergic R/ADRA2A Products

Product Documents for Human alpha ‑2A Adrenergic R/ADRA2A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human alpha ‑2A Adrenergic R/ADRA2A Antibody

For research use only

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