Human Angiopoietin-2 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY623
Ancillary Products Available
Human Angiopoietin-2 ELISA Standard Curve
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Product Details
Citations (22)
FAQs
Supplemental Products
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Human Angiopoietin-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human Angiopoietin-2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:
285 (Human); 11601 (Mouse)
Alternate Names:
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand

Citations for Human Angiopoietin-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study
    Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
    BMC Pediatr, 2018;18(1):118.
    Species: Human
    Sample Types: Plasma
  2. Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis
    Authors: J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David
    Sci Rep, 2017;7(0):44113.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Elevated cerebrospinal fluid tumor necrosis factor is associated with acute and long-term neurocognitive impairment in cerebral malaria
    Authors: E Shabani, BJ Ouma, R Idro, P Bangirana, RO Opoka, GS Park, AL Conroy, CC John
    Parasite Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  4. Plasmodium falciparum EPCR-binding PfEMP1 expression increases with malaria disease severity and is elevated in retinopathy negative cerebral malaria
    Authors: E Shabani, B Hanisch, RO Opoka, T Lavstsen, CC John
    BMC Med, 2017;15(1):183.
    Species: Human
    Sample Types: Plasma
  5. The endothelial protein C receptor rs867186-GG genotype is associated with increased soluble EPCR and could mediate protection against severe malaria
    Authors: Estela Shabani
    Sci Rep, 2016;6(0):27084.
    Species: Human
    Sample Types: Plasma
  6. Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
    Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
    PLoS Negl Trop Dis, 2015;9(6):e0003824.
    Species: Human
    Sample Types: Plasma
  7. Inhaled nitric oxide as adjunctive therapy for severe malaria: a randomized controlled trial.
    Authors: Hawkes M, Conroy A, Opoka R, Hermann L, Thorpe K, McDonald C, Kim H, Higgins S, Namasopo S, John C, Miller C, Liles W, Kain K
    Malar J, 2015;14(1):421.
    Species: Human
    Sample Types: Serum
  8. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015;10(8):e0134619.
    Species: Human
    Sample Types: Plasma
  9. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014;14(0):35.
    Species: Human
    Sample Types: Serum
  10. Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A.
    Am. J. Pathol., 2012;181(2):693-705.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Angiopoietin-2 Is a Direct Transcriptional Target of TAL1, LYL1 and LMO2 in Endothelial Cells.
    Authors: Deleuze V, El-Hajj R, Chalhoub E, Dohet C, Pinet V, Couttet P, Mathieu D
    PLoS ONE, 2012;7(7):e40484.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
    Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
    PLoS ONE, 2011;6(2):e17440.
    Species: Human
    Sample Types: Plasma
  13. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011;6(12):e28540.
    Species: Human
    Sample Types: Plasma
  14. Shedding of the endothelial receptor tyrosine kinase Tie2 correlates with leukemic blast burden and outcome after allogeneic hematopoietic stem cell transplantation for AML.
    Authors: Koenecke C, Kumpers P, Lukasz A, Dammann E, Verhagen W, Gohring G, Buchholz S, Krauter J, Eder M, Schlegelberger B, Ganser A
    Ann. Hematol., 2010;89(5):459-67.
    Species: Human
    Sample Types: Serum
  15. Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
    Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
    PLoS ONE, 2010;5(12):e15291.
    Species: Human
    Sample Types: Plasma
  16. Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
    Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
    Am. J. Hypertens., 2009;22(8):891-5.
    Species: Human
    Sample Types: Plasma
  17. Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
    Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
    Pathol. Oncol. Res., 2009;15(2):193-201.
    Species: Human
    Sample Types: Serum
  18. Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
    Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
    Thorax, 2008;63(10):903-9.
    Species: Human
    Sample Types: Plasma
  19. Angiopoietin-2 is increased in severe sepsis: correlation with inflammatory mediators.
    Authors: Orfanos SE, Kotanidou A, Glynos C, Athanasiou C, Tsigkos S, Dimopoulou I, Sotiropoulou C, Zakynthinos S, Armaganidis A, Papapetropoulos A, Roussos C
    Crit. Care Med., 2007;35(1):199-206.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  20. Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells.
    Authors: Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, Papapetropoulos A
    J. Cell. Physiol., 2006;207(2):506-11.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  21. Angiopoietin-2 levels are elevated in exudative pleural effusions.
    Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
    Chest, 2006;129(5):1259-66.
    Species: Human
    Sample Types: Serum
  22. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003;33(10):883-90.
    Species: Human
    Sample Types: Plasma

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