Human Angiopoietin-2 DuoSet ELISA

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Human Angiopoietin-2 ELISA Standard Curve
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Product Details
Citations (36)
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Human Angiopoietin-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
93.8 - 6,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Angiopoietin-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names:
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand

Citations for Human Angiopoietin-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department
    Authors: S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich
    Scientific Reports, 2022-05-24;12(1):8733.
    Species: Human
    Sample Types: Serum
  2. Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus
    Authors: M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand
    Cardiovascular Diabetology, 2022-05-12;21(1):72.
    Species: Human
    Sample Types: Plasma
  3. Clinical and biological markers for predicting ARDS and outcome in septic patients
    Authors: J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o
    Scientific Reports, 2021-11-22;11(1):22702.
    Species: Human
    Sample Types: Serum
  4. Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice
    Authors: NV Bogatcheva, ME Coleman
    Cells, 2021-03-24;10(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Early bilirubinemia after allogeneic stem cell transplantation-an endothelial complication
    Authors: H Dai, O Penack, A Radujkovic, D Schult, J Majer-Laut, IW Blau, L Bullinger, S Jiang, C Müller-Tid, P Dreger, T Luft
    Bone marrow transplantation, 2021-01-30;0(0):.
    Species: Human
    Sample Types: Serum
  6. Plasma angiopoietin-2 is associated with age-related deficits in cognitive sub-scales in Ugandan children following severe malaria
    Authors: BJ Ouma, P Bangirana, JM Ssenkusu, D Datta, RO Opoka, R Idro, KC Kain, CC John, AL Conroy
    Malaria Journal, 2021-01-06;20(1):17.
    Species: Human
    Sample Types: Plasma
  7. Exploring the potential effect of paricalcitol on markers of inflammation in de novo renal transplant recipients
    Authors: HK Pihlstrøm, T Ueland, AE Michelsen, P Aukrust, F Gatti, C Hammarströ, M Kasprzycka, J Wang, G Haraldsen, G Mjøen, DO Dahle, K Midtvedt, IA Eide, A Hartmann, H Holdaas
    PLoS ONE, 2020-12-16;15(12):e0243759.
    Species: Human
    Sample Types: Plasma
  8. Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment
    Authors: J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs
    Mar Drugs, 2020-09-21;18(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency
    Authors: A Chatterjee, R Eshwaran, H Huang, D Zhao, M Schmidt, T Wieland, Y Feng
    Int J Mol Sci, 2020-05-25;21(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes
    Authors: PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti
    Dis. Markers, 2020-01-13;2020(0):6163487.
    Species: Human
    Sample Types: Plasma
  11. EASIX for prediction of survival in lower-risk myelodysplastic syndromes
    Authors: A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft
    Blood Cancer J, 2019-11-11;9(11):85.
    Species: Human
    Sample Types: Plasma
  12. High baseline Tie1 level predicts poor survival in metastatic breast cancer
    Authors: L Tiainen, EA Korhonen, VM Leppänen, T Luukkaala, M Hämäläinen, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, S Aho, E Moilanen, K Alitalo, PL Kellokumpu
    BMC Cancer, 2019-07-24;19(1):732.
    Species: Human
    Sample Types: Plasma
  13. Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells
    Authors: H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada
    Reprod Biol, 2019-03-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Angiopoietin-2: A Potential Mediator of the Glycocalyx Injury in Adult Nephrotic Patients
    Authors: MMS Chaves, MS Mendes, MP Schwermann, R Queiroz, RF Coelho, FTS Salmito, GC Meneses, AMC Martins, ACOM Moreira, AB Libório
    J Clin Med, 2018-10-31;7(11):.
    Species: Human
    Sample Types: Plasma
  15. Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
    Authors: J Bourland, J Fradette, FA Auger
    Sci Rep, 2018-09-04;8(1):13191.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study
    Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
    BMC Pediatr, 2018-03-23;18(1):118.
    Species: Human
    Sample Types: Plasma
  17. Plasmodium falciparum EPCR-binding PfEMP1 expression increases with malaria disease severity and is elevated in retinopathy negative cerebral malaria
    Authors: E Shabani, B Hanisch, RO Opoka, T Lavstsen, CC John
    BMC Med, 2017-10-13;15(1):183.
    Species: Human
    Sample Types: Plasma
  18. Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis
    Authors: J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David
    Sci Rep, 2017-03-09;7(0):44113.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. The endothelial protein C receptor rs867186-GG genotype is associated with increased soluble EPCR and could mediate protection against severe malaria
    Authors: Estela Shabani
    Sci Rep, 2016-06-03;6(0):27084.
    Species: Human
    Sample Types: Plasma
  20. Inhaled nitric oxide as adjunctive therapy for severe malaria: a randomized controlled trial.
    Authors: Hawkes M, Conroy A, Opoka R, Hermann L, Thorpe K, McDonald C, Kim H, Higgins S, Namasopo S, John C, Miller C, Liles W, Kain K
    Malar J, 2015-10-29;14(1):421.
    Species: Human
    Sample Types: Serum
  21. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015-08-06;10(8):e0134619.
    Species: Human
    Sample Types: Plasma
  22. Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
    Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
    PLoS Negl Trop Dis, 2015-06-19;9(6):e0003824.
    Species: Human
    Sample Types: Plasma
  23. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014-01-20;14(0):35.
    Species: Human
    Sample Types: Serum
  24. Angiopoietin-2 Is a Direct Transcriptional Target of TAL1, LYL1 and LMO2 in Endothelial Cells.
    Authors: Deleuze V, El-Hajj R, Chalhoub E, Dohet C, Pinet V, Couttet P, Mathieu D
    PLoS ONE, 2012-07-06;7(7):e40484.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A.
    Am. J. Pathol., 2012-05-31;181(2):693-705.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011-12-12;6(12):e28540.
    Species: Human
    Sample Types: Plasma
  27. Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
    Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
    PLoS ONE, 2011-02-25;6(2):e17440.
    Species: Human
    Sample Types: Plasma
  28. Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
    Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
    PLoS ONE, 2010-12-29;5(12):e15291.
    Species: Human
    Sample Types: Plasma
  29. Shedding of the endothelial receptor tyrosine kinase Tie2 correlates with leukemic blast burden and outcome after allogeneic hematopoietic stem cell transplantation for AML.
    Authors: Koenecke C, Kumpers P, Lukasz A, Dammann E, Verhagen W, Gohring G, Buchholz S, Krauter J, Eder M, Schlegelberger B, Ganser A
    Ann. Hematol., 2010-05-01;89(5):459-67.
    Species: Human
    Sample Types: Serum
  30. Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
    Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
    Am. J. Hypertens., 2009-05-28;22(8):891-5.
    Species: Human
    Sample Types: Plasma
  31. Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
    Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
    Pathol. Oncol. Res., 2008-09-20;15(2):193-201.
    Species: Human
    Sample Types: Serum
  32. Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
    Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
    Thorax, 2008-06-17;63(10):903-9.
    Species: Human
    Sample Types: Plasma
  33. Angiopoietin-2 is increased in severe sepsis: correlation with inflammatory mediators.
    Authors: Orfanos SE, Kotanidou A, Glynos C, Athanasiou C, Tsigkos S, Dimopoulou I, Sotiropoulou C, Zakynthinos S, Armaganidis A, Papapetropoulos A, Roussos C
    Crit. Care Med., 2007-01-01;35(1):199-206.
    Species: Bovine, Human
    Sample Types: Cell Culture Supernates, Serum
  34. Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells.
    Authors: Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, Papapetropoulos A
    J. Cell. Physiol., 2006-05-01;207(2):506-11.
    Species: Bovine
    Sample Types: Cell Culture Supernates
  35. Angiopoietin-2 levels are elevated in exudative pleural effusions.
    Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
    Chest, 2006-05-01;129(5):1259-66.
    Species: Human
    Sample Types: Serum
  36. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
    Species: Human
    Sample Types: Plasma


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