Human Angiopoietin-2 DuoSet ELISA
1 Image
Summary
Product Features
Kit Content
Other Reagents Required
Data Examples
Product Datasheets
Preparation and Storage
Background
Human Angiopoietin-2 DuoSet ELISA Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
93.8 - 6,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Complete Your Research
Complete Your Experiment
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-2
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
Entrez Gene IDs:
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names:
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
Citations for Human Angiopoietin-2 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
37
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus
Authors: M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand
Cardiovascular Diabetology, 2022;21(1):72.
Species: Human
Sample Types: Plasma
-
No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department
Authors: S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich
Scientific Reports, 2022;12(1):8733.
Species: Human
Sample Types: Serum
-
Clinical and biological markers for predicting ARDS and outcome in septic patients
Authors: J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o
Scientific Reports, 2021;11(1):22702.
Species: Human
Sample Types: Serum
-
Early bilirubinemia after allogeneic stem cell transplantation-an endothelial complication
Authors: H Dai, O Penack, A Radujkovic, D Schult, J Majer-Laut, IW Blau, L Bullinger, S Jiang, C Müller-Tid, P Dreger, T Luft
Bone marrow transplantation, 2021;0(0):.
Species: Human
Sample Types: Serum
-
Plasma angiopoietin-2 is associated with age-related deficits in cognitive sub-scales in Ugandan children following severe malaria
Authors: BJ Ouma, P Bangirana, JM Ssenkusu, D Datta, RO Opoka, R Idro, KC Kain, CC John, AL Conroy
Malaria Journal, 2021;20(1):17.
Species: Human
Sample Types: Plasma
-
Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice
Authors: NV Bogatcheva, ME Coleman
Cells, 2021;10(4):.
Species: Human
Sample Types: Cell Culture Supernates
-
Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency
Authors: A Chatterjee, R Eshwaran, H Huang, D Zhao, M Schmidt, T Wieland, Y Feng
Int J Mol Sci, 2020;21(10):.
Species: Human
Sample Types: Cell Culture Supernates
-
Exploring the potential effect of paricalcitol on markers of inflammation in de novo renal transplant recipients
Authors: HK Pihlstrøm, T Ueland, AE Michelsen, P Aukrust, F Gatti, C Hammarströ, M Kasprzycka, J Wang, G Haraldsen, G Mjøen, DO Dahle, K Midtvedt, IA Eide, A Hartmann, H Holdaas
PLoS ONE, 2020;15(12):e0243759.
Species: Human
Sample Types: Plasma
-
Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment
Authors: J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs
Mar Drugs, 2020;18(9):.
Species: Human
Sample Types: Cell Culture Supernates
-
Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes
Authors: PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti
Dis. Markers, 2020;2020(0):6163487.
Species: Human
Sample Types: Plasma
-
EASIX for prediction of survival in lower-risk myelodysplastic syndromes
Authors: A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft
Blood Cancer J, 2019;9(11):85.
Species: Human
Sample Types: Plasma
-
High baseline Tie1 level predicts poor survival in metastatic breast cancer
Authors: L Tiainen, EA Korhonen, VM Leppänen, T Luukkaala, M Hämäläinen, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, S Aho, E Moilanen, K Alitalo, PL Kellokumpu
BMC Cancer, 2019;19(1):732.
Species: Human
Sample Types: Plasma
-
Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells
Authors: H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada
Reprod Biol, 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
Authors: J Bourland, J Fradette, FA Auger
Sci Rep, 2018;8(1):13191.
Species: Human
Sample Types: Cell Culture Supernates
-
Angiopoietin-2: A Potential Mediator of the Glycocalyx Injury in Adult Nephrotic Patients
Authors: MMS Chaves, MS Mendes, MP Schwermann, R Queiroz, RF Coelho, FTS Salmito, GC Meneses, AMC Martins, ACOM Moreira, AB Libório
J Clin Med, 2018;7(11):.
Species: Human
Sample Types: Plasma
-
Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study
Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
BMC Pediatr, 2018;18(1):118.
Species: Human
Sample Types: Plasma
-
Elevated cerebrospinal fluid tumor necrosis factor is associated with acute and long-term neurocognitive impairment in cerebral malaria
Authors: E Shabani, BJ Ouma, R Idro, P Bangirana, RO Opoka, GS Park, AL Conroy, CC John
Parasite Immunol., 2017;0(0):.
Species: Human
Sample Types: Plasma
-
Plasmodium falciparum EPCR-binding PfEMP1 expression increases with malaria disease severity and is elevated in retinopathy negative cerebral malaria
Authors: E Shabani, B Hanisch, RO Opoka, T Lavstsen, CC John
BMC Med, 2017;15(1):183.
Species: Human
Sample Types: Plasma
-
Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis
Authors: J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David
Sci Rep, 2017;7(0):44113.
Species: Human
Sample Types: Cell Culture Supernates
-
The endothelial protein C receptor rs867186-GG genotype is associated with increased soluble EPCR and could mediate protection against severe malaria
Authors: Estela Shabani
Sci Rep, 2016;6(0):27084.
Species: Human
Sample Types: Plasma
-
Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
PLoS ONE, 2015;10(8):e0134619.
Species: Human
Sample Types: Plasma
-
Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
PLoS Negl Trop Dis, 2015;9(6):e0003824.
Species: Human
Sample Types: Plasma
-
Inhaled nitric oxide as adjunctive therapy for severe malaria: a randomized controlled trial.
Authors: Hawkes M, Conroy A, Opoka R, Hermann L, Thorpe K, McDonald C, Kim H, Higgins S, Namasopo S, John C, Miller C, Liles W, Kain K
Malar J, 2015;14(1):421.
Species: Human
Sample Types: Serum
-
Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
BMC Infect Dis, 2014;14(0):35.
Species: Human
Sample Types: Serum
-
Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A.
Am. J. Pathol., 2012;181(2):693-705.
Species: Human
Sample Types: Cell Culture Supernates
-
Angiopoietin-2 Is a Direct Transcriptional Target of TAL1, LYL1 and LMO2 in Endothelial Cells.
Authors: Deleuze V, El-Hajj R, Chalhoub E, Dohet C, Pinet V, Couttet P, Mathieu D
PLoS ONE, 2012;7(7):e40484.
Species: Human
Sample Types: Cell Culture Supernates
-
Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
Authors: Conroy AL, Liles WC, Molyneux ME
PLoS ONE, 2011;6(12):e28540.
Species: Human
Sample Types: Plasma
-
Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
PLoS ONE, 2011;6(2):e17440.
Species: Human
Sample Types: Plasma
-
Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
PLoS ONE, 2010;5(12):e15291.
Species: Human
Sample Types: Plasma
-
Shedding of the endothelial receptor tyrosine kinase Tie2 correlates with leukemic blast burden and outcome after allogeneic hematopoietic stem cell transplantation for AML.
Authors: Koenecke C, Kumpers P, Lukasz A, Dammann E, Verhagen W, Gohring G, Buchholz S, Krauter J, Eder M, Schlegelberger B, Ganser A
Ann. Hematol., 2010;89(5):459-67.
Species: Human
Sample Types: Serum
-
Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
Am. J. Hypertens., 2009;22(8):891-5.
Species: Human
Sample Types: Plasma
-
Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
Pathol. Oncol. Res., 2009;15(2):193-201.
Species: Human
Sample Types: Serum
-
Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
Thorax, 2008;63(10):903-9.
Species: Human
Sample Types: Plasma
-
Angiopoietin-2 is increased in severe sepsis: correlation with inflammatory mediators.
Authors: Orfanos SE, Kotanidou A, Glynos C, Athanasiou C, Tsigkos S, Dimopoulou I, Sotiropoulou C, Zakynthinos S, Armaganidis A, Papapetropoulos A, Roussos C
Crit. Care Med., 2007;35(1):199-206.
Species: Bovine
Sample Types: Cell Culture Supernates
-
Angiopoietin-2 levels are elevated in exudative pleural effusions.
Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
Chest, 2006;129(5):1259-66.
Species: Human
Sample Types: Serum
-
Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells.
Authors: Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, Papapetropoulos A
J. Cell. Physiol., 2006;207(2):506-11.
Species: Bovine
Sample Types: Cell Culture Supernates
-
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
Eur. J. Clin. Invest., 2003;33(10):883-90.
Species: Human
Sample Types: Plasma
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsDuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Human Angiopoietin-2 DuoSet ELISA
There are currently no reviews for this product. Be the first to review Human Angiopoietin-2 DuoSet ELISA and earn rewards!
Have you used Human Angiopoietin-2 DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
