Human Angiopoietin-2 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Data Example
Product Datasheets
Preparation and Storage
Background: Angiopoietin-2
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
Citations for Human Angiopoietin-2 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
28
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes
Authors: PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti
Dis. Markers, 2020;2020(0):6163487.
Species: Human
Sample Types: Plasma
-
EASIX for prediction of survival in lower-risk myelodysplastic syndromes
Authors: A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft
Blood Cancer J, 2019;9(11):85.
Species: Human
Sample Types: Plasma
-
High baseline Tie1 level predicts poor survival in metastatic breast cancer
Authors: L Tiainen, EA Korhonen, VM Leppänen, T Luukkaala, M Hämäläinen, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, S Aho, E Moilanen, K Alitalo, PL Kellokumpu
BMC Cancer, 2019;19(1):732.
Species: Human
Sample Types: Plasma
-
Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells
Authors: H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada
Reprod Biol, 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
Authors: J Bourland, J Fradette, FA Auger
Sci Rep, 2018;8(1):13191.
Species: Human
Sample Types: Cell Culture Supernates
-
Angiopoietin-2: A Potential Mediator of the Glycocalyx Injury in Adult Nephrotic Patients
Authors: MMS Chaves, MS Mendes, MP Schwermann, R Queiroz, RF Coelho, FTS Salmito, GC Meneses, AMC Martins, ACOM Moreira, AB Libório
J Clin Med, 2018;7(11):.
Species: Human
Sample Types: Plasma
-
Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study
Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
BMC Pediatr, 2018;18(1):118.
Species: Human
Sample Types: Plasma
-
Plasmodium falciparum EPCR-binding PfEMP1 expression increases with malaria disease severity and is elevated in retinopathy negative cerebral malaria
Authors: E Shabani, B Hanisch, RO Opoka, T Lavstsen, CC John
BMC Med, 2017;15(1):183.
Species: Human
Sample Types: Plasma
-
Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis
Authors: J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David
Sci Rep, 2017;7(0):44113.
Species: Human
Sample Types: Cell Culture Supernates
-
Elevated cerebrospinal fluid tumor necrosis factor is associated with acute and long-term neurocognitive impairment in cerebral malaria
Authors: E Shabani, BJ Ouma, R Idro, P Bangirana, RO Opoka, GS Park, AL Conroy, CC John
Parasite Immunol., 2017;0(0):.
Species: Human
Sample Types: Plasma
-
The endothelial protein C receptor rs867186-GG genotype is associated with increased soluble EPCR and could mediate protection against severe malaria
Authors: Estela Shabani
Sci Rep, 2016;6(0):27084.
Species: Human
Sample Types: Plasma
-
Inhaled nitric oxide as adjunctive therapy for severe malaria: a randomized controlled trial.
Authors: Hawkes M, Conroy A, Opoka R, Hermann L, Thorpe K, McDonald C, Kim H, Higgins S, Namasopo S, John C, Miller C, Liles W, Kain K
Malar J, 2015;14(1):421.
Species: Human
Sample Types: Serum
-
Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
PLoS Negl Trop Dis, 2015;9(6):e0003824.
Species: Human
Sample Types: Plasma
-
Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
PLoS ONE, 2015;10(8):e0134619.
Species: Human
Sample Types: Plasma
-
Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
BMC Infect Dis, 2014;14(0):35.
Species: Human
Sample Types: Serum
-
Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A.
Am. J. Pathol., 2012;181(2):693-705.
Species: Human
Sample Types: Cell Culture Supernates
-
Angiopoietin-2 Is a Direct Transcriptional Target of TAL1, LYL1 and LMO2 in Endothelial Cells.
Authors: Deleuze V, El-Hajj R, Chalhoub E, Dohet C, Pinet V, Couttet P, Mathieu D
PLoS ONE, 2012;7(7):e40484.
Species: Human
Sample Types: Cell Culture Supernates
-
Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
PLoS ONE, 2011;6(2):e17440.
Species: Human
Sample Types: Plasma
-
Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
Authors: Conroy AL, Liles WC, Molyneux ME
PLoS ONE, 2011;6(12):e28540.
Species: Human
Sample Types: Plasma
-
Shedding of the endothelial receptor tyrosine kinase Tie2 correlates with leukemic blast burden and outcome after allogeneic hematopoietic stem cell transplantation for AML.
Authors: Koenecke C, Kumpers P, Lukasz A, Dammann E, Verhagen W, Gohring G, Buchholz S, Krauter J, Eder M, Schlegelberger B, Ganser A
Ann. Hematol., 2010;89(5):459-67.
Species: Human
Sample Types: Serum
-
Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
PLoS ONE, 2010;5(12):e15291.
Species: Human
Sample Types: Plasma
-
Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
Am. J. Hypertens., 2009;22(8):891-5.
Species: Human
Sample Types: Plasma
-
Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
Pathol. Oncol. Res., 2009;15(2):193-201.
Species: Human
Sample Types: Serum
-
Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
Thorax, 2008;63(10):903-9.
Species: Human
Sample Types: Plasma
-
Angiopoietin-2 is increased in severe sepsis: correlation with inflammatory mediators.
Authors: Orfanos SE, Kotanidou A, Glynos C, Athanasiou C, Tsigkos S, Dimopoulou I, Sotiropoulou C, Zakynthinos S, Armaganidis A, Papapetropoulos A, Roussos C
Crit. Care Med., 2007;35(1):199-206.
Species: Bovine
Sample Types: Cell Culture Supernates
-
Angiopoietin-2 levels are elevated in exudative pleural effusions.
Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
Chest, 2006;129(5):1259-66.
Species: Human
Sample Types: Serum
-
Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells.
Authors: Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, Papapetropoulos A
J. Cell. Physiol., 2006;207(2):506-11.
Species: Bovine
Sample Types: Cell Culture Supernates
-
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
Eur. J. Clin. Invest., 2003;33(10):883-90.
Species: Human
Sample Types: Plasma
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsDuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Human Angiopoietin-2 DuoSet ELISA
There are currently no reviews for this product. Be the first to review Human Angiopoietin-2 DuoSet ELISA and earn rewards!
Have you used Human Angiopoietin-2 DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image