Human Angiopoietin-2 DuoSet ELISA

DY623 28 Citations Datasheet / COA / SDS

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DY008: DuoSet ELISA Ancillary Reagent Kit 2

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Human Angiopoietin-2 ELISA Standard Curve

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Citations (28)

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Angiopoietin-2 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

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Human Angiopoietin-2 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:

285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)

Alternate Names:

AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand

Citations for Human Angiopoietin-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes
Authors: PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti
Dis. Markers, 2020;2020(0):6163487.
Species: Human
Sample Types: Plasma
EASIX for prediction of survival in lower-risk myelodysplastic syndromes
Authors: A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft
Blood Cancer J, 2019;9(11):85.
Species: Human
Sample Types: Plasma
High baseline Tie1 level predicts poor survival in metastatic breast cancer
Authors: L Tiainen, EA Korhonen, VM Leppänen, T Luukkaala, M Hämäläinen, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, S Aho, E Moilanen, K Alitalo, PL Kellokumpu
BMC Cancer, 2019;19(1):732.
Species: Human
Sample Types: Plasma
Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells
Authors: H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada
Reprod Biol, 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
Authors: J Bourland, J Fradette, FA Auger
Sci Rep, 2018;8(1):13191.
Species: Human
Sample Types: Cell Culture Supernates
Angiopoietin-2: A Potential Mediator of the Glycocalyx Injury in Adult Nephrotic Patients
Authors: MMS Chaves, MS Mendes, MP Schwermann, R Queiroz, RF Coelho, FTS Salmito, GC Meneses, AMC Martins, ACOM Moreira, AB Libório
J Clin Med, 2018;7(11):.
Species: Human
Sample Types: Plasma
Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matchedï¿½case-control study
Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
BMC Pediatr, 2018;18(1):118.
Species: Human
Sample Types: Plasma
Plasmodium falciparum EPCR-binding PfEMP1 expression increases with malaria disease severity and is elevated in retinopathy negative cerebral malaria
Authors: E Shabani, B Hanisch, RO Opoka, T Lavstsen, CC John
BMC Med, 2017;15(1):183.
Species: Human
Sample Types: Plasma
Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis
Authors: J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David
Sci Rep, 2017;7(0):44113.
Species: Human
Sample Types: Cell Culture Supernates
Elevated cerebrospinal fluid tumor necrosis factor is associated with acute and long-term neurocognitive impairment in cerebral malaria
Authors: E Shabani, BJ Ouma, R Idro, P Bangirana, RO Opoka, GS Park, AL Conroy, CC John
Parasite Immunol., 2017;0(0):.
Species: Human
Sample Types: Plasma

The endothelial protein C receptor rs867186-GG genotype is associated with increased soluble EPCR and could mediate protection against severe malaria
Authors: Estela Shabani
Sci Rep, 2016;6(0):27084.
Species: Human
Sample Types: Plasma

Inhaled nitric oxide as adjunctive therapy for severe malaria: a randomized controlled trial.
Authors: Hawkes M, Conroy A, Opoka R, Hermann L, Thorpe K, McDonald C, Kim H, Higgins S, Namasopo S, John C, Miller C, Liles W, Kain K
Malar J, 2015;14(1):421.
Species: Human
Sample Types: Serum

Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
PLoS Negl Trop Dis, 2015;9(6):e0003824.
Species: Human
Sample Types: Plasma

Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
PLoS ONE, 2015;10(8):e0134619.
Species: Human
Sample Types: Plasma

Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
BMC Infect Dis, 2014;14(0):35.
Species: Human
Sample Types: Serum

Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A.
Am. J. Pathol., 2012;181(2):693-705.
Species: Human
Sample Types: Cell Culture Supernates

Angiopoietin-2 Is a Direct Transcriptional Target of TAL1, LYL1 and LMO2 in Endothelial Cells.
Authors: Deleuze V, El-Hajj R, Chalhoub E, Dohet C, Pinet V, Couttet P, Mathieu D
PLoS ONE, 2012;7(7):e40484.
Species: Human
Sample Types: Cell Culture Supernates

Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
PLoS ONE, 2011;6(2):e17440.
Species: Human
Sample Types: Plasma

Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
Authors: Conroy AL, Liles WC, Molyneux ME
PLoS ONE, 2011;6(12):e28540.
Species: Human
Sample Types: Plasma

Shedding of the endothelial receptor tyrosine kinase Tie2 correlates with leukemic blast burden and outcome after allogeneic hematopoietic stem cell transplantation for AML.
Authors: Koenecke C, Kumpers P, Lukasz A, Dammann E, Verhagen W, Gohring G, Buchholz S, Krauter J, Eder M, Schlegelberger B, Ganser A
Ann. Hematol., 2010;89(5):459-67.
Species: Human
Sample Types: Serum

Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
PLoS ONE, 2010;5(12):e15291.
Species: Human
Sample Types: Plasma

Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
Am. J. Hypertens., 2009;22(8):891-5.
Species: Human
Sample Types: Plasma

Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
Pathol. Oncol. Res., 2009;15(2):193-201.
Species: Human
Sample Types: Serum

Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
Thorax, 2008;63(10):903-9.
Species: Human
Sample Types: Plasma

Angiopoietin-2 is increased in severe sepsis: correlation with inflammatory mediators.
Authors: Orfanos SE, Kotanidou A, Glynos C, Athanasiou C, Tsigkos S, Dimopoulou I, Sotiropoulou C, Zakynthinos S, Armaganidis A, Papapetropoulos A, Roussos C
Crit. Care Med., 2007;35(1):199-206.
Species: Bovine
Sample Types: Cell Culture Supernates

Angiopoietin-2 levels are elevated in exudative pleural effusions.
Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
Chest, 2006;129(5):1259-66.
Species: Human
Sample Types: Serum

Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells.
Authors: Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, Papapetropoulos A
J. Cell. Physiol., 2006;207(2):506-11.
Species: Bovine
Sample Types: Cell Culture Supernates

Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
Eur. J. Clin. Invest., 2003;33(10):883-90.
Species: Human
Sample Types: Plasma