Human APRIL/TNFSF13 Antibody

  (2 citations)     
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  • Species Reactivity
    Human
  • Specificity
    Detects human APRIL/TNFSF13 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse APRIL is observed, and no cross-reactivity with recombinant human BAFF is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 670820
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human embryonic kidney cell line HEK293-derived recombinant human APRIL/TNFSF13
    Ala105-Leu250
    Accession # O75888
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize APRIL/TNFSF13-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 0.15-0.75 ug/mL in the presence of 200 ng/mL Recombinant Human APRIL/TNFSF13.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Cell Proliferation Induced by APRIL/TNFSF13 and Neutralization by Human APRIL/TNFSF13 Antibody. Recombinant Human APRIL/TNFSF13 induces proliferation in mouse B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND50 is typically
0.15-0.75 µg/mL.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APRIL/TNFSF13
APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.
  • Long Name:
    A Proliferation-inducing Ligand
  • Entrez Gene IDs:
    8741 (Human); 69583 (Mouse)
  • Alternate Names:
    A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Nurse-like cells promote CLL survival through LFA-3/CD2 interactions
    Oncotarget, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL.
    Authors: Osorio C, Chacon P, White M, Kisiswa L, Wyatt S, Rodriguez-Tebar A, Davies A
    Mol Cell Neurosci, 2014;59(0):24-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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Isotype Controls
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Mouse IgG1 Isotype Control

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Reconstitution Buffer 1 (PBS)

RB01
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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