Detects human APRIL/TNFSF13 in direct ELISAs.
In direct ELISAs, approximately 10%
cross-reactivity with recombinant mouse APRIL is observed, and no
cross-reactivity with recombinant human BAFF is observed.
Monoclonal Mouse IgG1 Clone # 670820
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell line HEK293-derived recombinant human APRIL/TNFSF13 Ala105-Leu250 Accession # O75888
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize APRIL/TNFSF13-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 0.15-0.75 ug/mL in the presence of 200 ng/mL Recombinant Human APRIL/TNFSF13.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by APRIL/TNFSF13 and Neutralization by Human APRIL/TNFSF13 Antibody.
Recombinant Human APRIL/TNFSF13 induces proliferation in mouse B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND50 is typically 0.15-0.75 µg/mL.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.
A Proliferation-inducing Ligand
Entrez Gene IDs:
8741 (Human); 69583 (Mouse)
A proliferation-inducing ligand; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.