Human ARX Antibody

Catalog # Availability Size / Price Qty
Detection of Human ARX by Western Blot.
3 Images
Product Details
Citations (3)
Supplemental Products

Human ARX Antibody Summary

Species Reactivity
Detects human ARX in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human ARX
Accession # Q96QS3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
5-15 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ARX antibody by Western Blot. View Larger

Detection of Human ARX by Western Blot. Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ARX antibody in U-87 MG Human Cell Line by Immunocytochemistry (ICC). View Larger

ARX in U‑87 MG Human Cell Line. ARX was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ARX antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

ARX in Human Pancreas. ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARX

ARX (Aristaless-Related homeoboX protein) is a 56-62 kDa member of the aristaless subfamily, paired homeobox family of proteins. In the nervous system, it is expressed in embryonic telencephalon plus adult cortical GABAergic neurons and differentiating neuronal stem cells; in the pancreas, it promotes the differentiation of alpha -cells at the expense of beta -cells. Human ARX is a 562 amino acid (aa) transcriptional repressor. It contains an octapeptide motif that binds Groucho proteins (aa 27‑34), an NLS (aa 82-89), one Ala-rich region (aa 100-155), an acidic/Glu-rich region (aa 224-253), a DNA-binding homeodomain with an embedded NLS (aa 328‑387), one Pro-rich segment that binds CtBP1 (aa 395-459) and a C-terminal Aristaless domain (aa 530-543). There are mutations that show poly-Ala expansions in the N-terminus (aa 115-155), often associated with developmental abnormalities. Over aa 1-100, human ARX shares 97% aa identity with mouse ARX.

Long Name
Aristless-related Homeobox Protein
Entrez Gene IDs
170302 (Human); 11878 (Mouse); 317268 (Rat)
Alternate Names
aristaless related homeobox; Aristaless-related homeobox; aristaless-related homeobox, X-linked; ARX; cancer/testis antigen 121; CT121; EIEE1; homeobox protein ARX; ISSX; ISSXmental retardation, X-linked 36; mental retardation, X-linked 29; mental retardation, X-linked 32; mental retardation, X-linked 33; mental retardation, X-linked 38; mental retardation, X-linked 43; mental retardation, X-linked 54; mental retardation, X-linked 76; mental retardation, X-linked 87; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRX76; MRX87; MRXS1; PRTS

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Citations for Human ARX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)
    Authors: N Moya, A Shahryari, I Burtscher, J Beckenbaue, M Bakhti, H Lickert
    Stem Cell Res, 2020;46(0):101874.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein
    Authors: X Shi, W Lin, X Gao, W Xie, JA Golden, T Tao
    Biosci. Rep., 2020;40(7):.
    Species: Human
    Sample Types: Cell culture supernate
  3. Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced
    Authors: P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren
    J Pathol Clin Res, 2019;5(4):248-255.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


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