Human ASCL2/Mash2 Antibody

Catalog # Availability Size / Price Qty
AF6539
AF6539-SP
Human ASCL2/Mash2 Antibody in Western Blot
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Human ASCL2/Mash2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ASCL2/Mash2 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ASCL2/Mash2
Met1-Ala49
Accession # Q99929
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human ASCL2/Mash2 by Western Blot. View Larger

Detection of Human ASCL2/Mash2 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASCL2/Mash2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry ASCL2/Mash2 in Human Intestine. View Larger

ASCL2/Mash2 in Human Intestine. ASCL2/Mash2 was detected in immersion fixed paraffin-embedded sections of human intestine using Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to the base of intestinal crypts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASCL2/Mash2

ASCL2 (Achaete-scute like 2; also HASH2, bHLHa45 and Mash2) is a class II member of the HLH family of transcription factors. Its predicted MW is 20 kDa. ASCL2 shows restricted expression, being limited to intestinal Lgr5+ stem cells and first trimester placental cytotrophoblasts. In the intestine, ASCL2 is under the control of Wnts and serves to maintain an epithelium stem cell pool. In the placenta, ASCL2 acts to maintain the pool of cytotrophoblasts at the expense of synctiotrophoblasts, thus promoting placental growth. Human ASCL2 is 193 amino acids (aa) in length. It contains one poly-Arg motif (aa 36-39), a DNA binding sequence (aa 53-63) and an HLH domain (aa 64-103). ASCL2 forms heterodimers with daughterless homologs (E22, E2A and HEB). Over aa 1-49, human ASCL2 shares 59% aa identity with mouse Mash2.

 

Long Name
Achaete-scute Complex-like 1
Entrez Gene IDs
430 (Human); 17173 (Mouse); 24209 (Rat)
Alternate Names
achaete-scute complex (Drosophila) homolog-like 2; achaete-scute complex homolog 2 (Drosophila); achaete-scute complex-like 2 (Drosophila); ASCL2; ASH2; ASH-2; BHLHA45; bHLHa45achaete-scute homolog 2; Class A basic helix-loop-helix protein 45; HASH2; HASH2achaete-scute complex-like 2; mammalian achaete/scute homologue 2; Mash2

Product Datasheets

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ASCL2/Mash2 Antibody

Average Rating: 2 (Based on 1 Review)

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Human ASCL2/Mash2 Antibody
By Anonymous on 08/23/2019
Application: ICC/IF Sample Tested: airway cells Species: Human

did not work with citrate retrieval - not sure if the signal is real