Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ASCL2/Mash2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ASCL2/Mash2
Met1-Ala49
Accession # Q99929

Specificity

Detects human ASCL2/Mash2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ASCL2/Mash2 Antibody

Detection of Human ASCL2/Mash2 antibody by Western Blot.

Detection of Human ASCL2/Mash2 by Western Blot.

Western blot shows lysates of JAR human choriocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for ASCL2/Mash2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of ASCL2/Mash2 in Human Placenta.

ASCL2/Mash2 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in extravillous trophoblast. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of ASCL2/Mash2 by Western Blot

Detection of ASCL2/Mash2 by Western Blot

A ASCL2, TLR4, NF-kappa B, IL-1 beta, and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-kappa B, IL-1 beta, and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, *P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,.#P < 0.05) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36008864), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ASCL2/Mash2 by Western Blot

Detection of ASCL2/Mash2 by Western Blot

A ASCL2, TLR4, NF-kappa B, IL-1 beta, and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-kappa B, IL-1 beta, and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, *P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,.#P < 0.05) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36008864), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ASCL2/Mash2 by Western Blot

Detection of ASCL2/Mash2 by Western Blot

A ASCL2, TLR4, NF-kappa B, IL-1 beta, and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-kappa B, IL-1 beta, and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, *P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,.#P < 0.05) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36008864), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ASCL2/Mash2 by Western Blot

Detection of ASCL2/Mash2 by Western Blot

A ASCL2, TLR4, NF-kappa B, IL-1 beta, and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-kappa B, IL-1 beta, and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, *P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,.#P < 0.05) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36008864), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human ASCL2/Mash2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Placenta

Western Blot

1 µg/mL
Sample: JAR human choriocarcinoma cell line and MCF7 human breast cancer cell line

Reviewed Applications

Read 2 reviews rated 3 using AF6539 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ASCL2/Mash2

ASCL2 (Achaete-scute like 2; also HASH2, bHLHa45 and Mash2) is a class II member of the HLH family of transcription factors. Its predicted MW is 20 kDa. ASCL2 shows restricted expression, being limited to intestinal Lgr5+ stem cells and first trimester placental cytotrophoblasts. In the intestine, ASCL2 is under the control of Wnts and serves to maintain an epithelium stem cell pool. In the placenta, ASCL2 acts to maintain the pool of cytotrophoblasts at the expense of synctiotrophoblasts, thus promoting placental growth. Human ASCL2 is 193 amino acids (aa) in length. It contains one poly-Arg motif (aa 36-39), a DNA binding sequence (aa 53-63) and an HLH domain (aa 64-103). ASCL2 forms heterodimers with daughterless homologs (E22, E2A and HEB). Over aa 1-49, human ASCL2 shares 59% aa identity with mouse Mash2.

Long Name

Achaete-scute Complex-like 1

Alternate Names

ASH2, BHLHA45, HASH2, Mash2

Entrez Gene IDs

430 (Human); 17173 (Mouse); 24209 (Rat)

Gene Symbol

ASCL2

UniProt

Q99929 (Human)

Additional ASCL2/Mash2 Products

Product Documents for Human ASCL2/Mash2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ASCL2/Mash2 Antibody

For research use only

Citations for Human ASCL2/Mash2 Antibody

Customer Reviews for Human ASCL2/Mash2 Antibody (2)

3 out of 5
2 Customer Ratings
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  • Human ASCL2/Mash2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 11/17/2021
    Human ASCL2/Mash2 Antibody AF6539
  • Human ASCL2/Mash2 Antibody
    Name: avinash waghray
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: airway cells
    Species: Human
    Verified Customer | Posted 08/23/2019
    did not work with citrate retrieval - not sure if the signal is real
    Human ASCL2/Mash2 Antibody AF6539

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Protocols

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