Human Attractin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Attractin in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Attractin
    Ala84-Gln1272
    Accession # NP_647538
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Attractin by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Attractin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7238) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Attractin at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Attractin in HepG2 Human Cell Line. Attractin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human Attractin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7238) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Attractin

Attractin (ATRN), also known as DPPT-L, is an approximately 200 kDa transmembrane glycoprotein that shows dipeptidyl peptidase activity similar to DPPIV/CD26. Attractin is involved in a variety of processes including monocyte-T cell adhesion, axon myelination, melanocyte pigment synthesis, and energy homeostasis. The extracellular region of human Attractin contains one EGF-like domain, one CUB domain, six Kelch repeats, four PSI domains, one C-type lectin domain, and two laminin EGF-like domains. Alternate splicing of human Attractin generates a secreted isoform that lacks the transmembrane and cyotplasmic regions. Attractin is transiently upregulated during T cell activation before expression switches to the 175 kDa secreted isoform which is released into the circulation. Soluble Attractin is preferentially expressed by leukocytes and differentiating neurons. It blocks neurite formation and is elevated in the CSF of astrocytoma patients. Within aa 84-1272, human Attractin shares 95% aa sequence identity with mouse and rat Attractin.

  • Entrez Gene IDs:
    8455 (Human); 11990 (Mouse); 83526 (Rat)
  • Alternate Names:
    ATRN; Attractin; DPPT-L; KIAA0548; Mahogany Protein; MGC126754; MGCA
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