Human B7-1/CD80 Fluorescein-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 37711
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80
    Val35-Asn242 (predicted)
    Accession # P33681
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB140F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-1/CD80
B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequence identity. Human and mouse B7-1 share 44% aa sequence identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.
  • References:
    1. Azuma, M. et al. (1993) Nature 366:76.
    2. Freeman, G.J. et al. (1993) Science 262:909.
    3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
    4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
    5. Chen, C. et al. (1994) J. Immunol. 152:4929.
    6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  • Entrez Gene IDs:
    941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
  • Alternate Names:
    Activation B7-1 antigen; B7; B71; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors.
    Authors: Petterson T, Jendholm J, Mansson A, Bjartell A, Riesbeck K, Cardell LO
    J. Leukoc. Biol., 2011;89(2):177-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells.
    Authors: Kraetzel K, Stoelcker B, Eissner G, Multhoff G, Pfeifer M, Holler E, Schulz C
    Eur. Respir. J., 2008;32(3):563-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates.
    Authors: Kaur K, Chowdhury S, Greenspan NS, Schreiber JR
    Blood, 2007;110(8):2948-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 9  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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