Human B7-1/CD80 Antibody MAB140-100: R&D Systems

Human B7-1/CD80 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 37711
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80
    Val35-Asn242 (predicted)
    Accession # P33681
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human B7-1/CD80 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human B7‑1/CD80 Antibody (Catalog # MAB140)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human B7‑1/CD80 Biotinylated Antibody (Catalog # BAM1402)
  • ELISA Standard
     
    Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF (Catalog # 140-B1)
  • Neutralization
    Measured by its ability to neutralize B7‑1/CD80 and PHA-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera and 10 µg/mL PHA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).
IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Human B7‑1/CD80 Antibody.

Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Human B7‑1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND50 is typically 0.2-1 µg/mL.

Immunohistochemistry
B7‑1/CD80 in Human Tonsil. B7‑1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through Interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequenceidentity. Human and mouse B7-1 share 44% aa sequenceidentity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

  • References:
    1. Azuma, M. et al. (1993) Nature 366:76.
    2. Freeman, G.J. et al. (1993) Science 262:909.
    3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
    4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
    5. Chen, C. et al. (1994) J. Immunol. 152:4929.
    6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  • Entrez Gene IDs:
    941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
  • Alternate Names:
    Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Tubular B7-1 expression parallels proteinuria levels, but not clinical outcomes in adult minimal change disease patients
    Authors: SW Lee, SH Baek, JH Paik, S Kim, KY Na, DW Chae, HJ Chin
    Sci Rep, 2017;7(0):41859.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  2. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014;2014(0):593562.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  3. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood, 2012;119(26):6268-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. B7-CD28 costimulatory signals control the survival and proliferation of murine and human gammadelta T cells via IL-2 production.
    J. Immunol., 2012;189(3):1202-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. A synergistic antitumor effect of interleukin-2 addition with CD80 immunogene therapy for peritoneal metastasis of gastric carcinoma.
    Authors: Kosaka K, Yashiro M, Sakate Y, Hirakawa K
    Dig. Dis. Sci., 2007;52(8):1946-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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