Human BAI2 Antibody Summary
Accession # O60241-3
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human BAI2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human BAI2. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human BAI2 Monoclonal Antibody (Catalog # MAB9338) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BAI2 at approximately 172 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of BAI2 in HEK293 Human Cell Line Transfected with Human BAI2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human BAI2 or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human BAI2 Monoclonal Antibody (Catalog # MAB9338) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).
- Duman, J.G. et al. (2016) Neural Plast. 2016:8301737.
- Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
- Kee, H.J. et al. (2002) J. Cereb. Blood Flow Metab. 22:1054.
- Daisuke, O. et al. (2010) J. Recept Signal Transduct Res 30:143.
- Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
- Jeong, B.C. et al. (2016) FEBS Lett 580:669.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human BAI2 Antibody
There are currently no reviews for this product. Be the first to review Human BAI2 Antibody and earn rewards!
Have you used Human BAI2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image