Human BAI2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Human BAI2 by Western Blot.
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Product Details
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Human BAI2 Antibody Summary

Species Reactivity
Detects human BAI2 in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2528B
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell, HEK293-derived human BAI2
Accession # O60241-3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BAI2 antibody by Western Blot. View Larger

Detection of Human BAI2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human BAI2. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human BAI2 Monoclonal Antibody (Catalog # MAB9338) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BAI2 at approximately 172 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of BAI2 antibody in HEK293 Human Cell Line Transfected with Human BAI2 and eGFP antibody by Flow Cytometry. View Larger

Detection of BAI2 in HEK293 Human Cell Line Transfected with Human BAI2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human BAI2 or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human BAI2 Monoclonal Antibody (Catalog # MAB9338) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAI2

Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).

  1. Duman, J.G. et al. (2016) Neural Plast. 2016:8301737.
  2. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
  3. Kee, H.J. et al. (2002) J. Cereb. Blood Flow Metab. 22:1054.
  4. Daisuke, O. et al. (2010) J. Recept Signal Transduct Res 30:143.
  5. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  6. Jeong, B.C. et al. (2016) FEBS Lett 580:669.
Long Name
Brain-specific Angiogenesis Inhibitor 2
Entrez Gene IDs
576 (Human); 230775 (Mouse); 313058 (Rat)
Alternate Names
BAI2; brain-specific angiogenesis inhibitor 2; Brain-specific angiongenesis inhibitor-2

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