Human BAI3 (brain-specific angiogenesis inhibitor 3) is a 177 kDa, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25‑883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296‑508), and one GPS domain (aa 816‑867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643‑665. Over aa 25‑880, human BAI3 shares 98% aa identity with mouse BAI3.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 409611
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BAI3
Ala25-Thr880
Accession # O60242
Ala25-Thr880
Accession # O60242
Specificity
Detects human BAI3 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human BAI3 Antibody
Detection of BAI3 in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human BAI3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human BAI3 Monoclonal Antibody (Catalog # MAB3965) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.Applications for Human BAI3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human BAI3 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human BAI3 and eGFP
Western Blot
1 µg/mL
Sample: Recombinant Human BAI3 under non-reducing conditions only
Sample: Recombinant Human BAI3 under non-reducing conditions only
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: BAI3
Long Name
Brain-specific Angiogenesis Inhibitor 3
Alternate Names
brain-specific angiogenesis inhibitor 3, dJ91B17.1 (brain-specific angiogenesis inhibitor 3), KIAA0550MGC133100
Gene Symbol
ADGRB3
UniProt
Additional BAI3 Products
Product Documents for Human BAI3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human BAI3 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Flow Cytometry Troubleshooting Guide
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- Protocol: Annexin V and PI Staining by Flow Cytometry
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- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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