Human Bcl-w Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Bcl-w in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 90316
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Bcl-w
    Met1-Thr172
    Accession # Q92843
  • Formulation
    Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Bcl‑w by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. The PVDF membrane was probed with 1 µg/mL Human Bcl‑w Monoclonal Antibody (Catalog # MAB824) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Bcl‑w Minus C‑Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9 with 25 mM Tris and 0.15 M NaCl.
Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Bcl-w

Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

 

  • References:
    1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
    2. Kroemer, G. (1997) Nature Med. 3:614.
    3. Ross, J.A., et al. (1998) Nat. Genet. 18:251.
    4. Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
    5. Yan, W. et al. (2000) Mol. Endocrin. 14:682.
  • Long Name:
    B Cell Lymphoma/Leukemia w
  • Entrez Gene IDs:
    599 (Human); 12050 (Mouse)
  • Alternate Names:
    Apoptosis regulator Bcl-W; BCL2L2; Bcl2-L-2; BCL2-like 2; Bclw; Bcl-w; BCLWbcl-2-like protein 2; KIAA0271BCL2-L-2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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