Detection of Human Bcl‑w by Western Blot.
Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. The PVDF membrane was probed with 1 µg/mL Human Bcl‑w Monoclonal Antibody (Catalog # MAB824) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Bcl‑w Minus C‑Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9 with 25 mM Tris and 0.15 M NaCl.
Preparation and Storage
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
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Kroemer, G. (1997) Nature Med. 3:614.
Ross, J.A., et al. (1998) Nat. Genet. 18:251.
Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
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