Human beta -1 Adrenergic R/ADRB1 Antibody Summary
Accession # P08588
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human beta ‑1 Adrenergic R/ADRB1 by Western Blot. Western blot shows lysates of human heart ventricle tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human beta -1 Adrenergic R/ADRB1 Monoclonal Antibody (Catalog # MAB10119) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for beta -1 Adrenergic R/ADRB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-1 Adrenergic R/ADRB1
The beta-1 Adrenergic Receptor (ADRB1) is a 477 aminoacids long and seven-transmembrane domain protein that belongs to the G protein-coupled receptor (GPCR) 1 family. The adrenergic receptors, including ADRB1, are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. ADRB1 binds epinephrine and norepinephrine with approximately equal affinity. ADRB1 is localized at the plasma membrane. It is also found in the Golgi upon Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) overexpression.
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