Human beta -1 Adrenergic R/ADRB1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10119-100
MAB10119-SP
Human  beta -1 Adrenergic R/ADRB1 Antibody in Western Blot
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Human beta -1 Adrenergic R/ADRB1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta ‑1 Adrenergic R/ADRB1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2203D
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human beta ‑1 Adrenergic R/ADRB1
Accession # P08588
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human  beta ‑1 Adrenergic R/ADRB1 by Western Blot. View Larger

Detection of Human beta ‑1 Adrenergic R/ADRB1 by Western Blot. Western blot shows lysates of human heart ventricle tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human beta -1 Adrenergic R/ADRB1 Monoclonal Antibody (Catalog # MAB10119) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for beta -1 Adrenergic R/ADRB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1 Adrenergic R/ADRB1

The beta-1 Adrenergic Receptor (ADRB1) is a 477 aminoacids long and seven-transmembrane domain protein that belongs to the G protein-coupled receptor (GPCR) 1 family. The adrenergic receptors, including ADRB1, are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. ADRB1 binds epinephrine and norepinephrine with approximately equal affinity. ADRB1 is localized at the plasma membrane. It is also found in the Golgi upon Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) overexpression.

Long Name
Beta-1 Adrenergic Receptor
Entrez Gene IDs
153 (Human); 11554 (Mouse); 24925 (Rat)
Alternate Names
ADRB1; ADRB1R; ADRB1RRHR; adrenergic, beta-1-, receptor; B1AR; beta-1 Adrenergic R; beta-1 adrenergic receptor; beta1 AdrenergicR; beta-1 AdrenergicR; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; BETA1AR

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