Human BPIFB1 Antibody

Catalog #: MAB101951 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB101951-100
MAB101951-SP
Human BPIFB1 ELISA Standard Curve.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human BPIFB1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BPIFB1 in sandwich ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1023925
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell HEK293-derived human BPIFB1
Thr22-Gln484
Accession # Q8TDL5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human BPIFB1 Monoclonal Antibody (Catalog # MAB101952).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human BPIFB1 ELISA Standard Curve. Recombinant Human BPIFB1 protein was serially diluted 2-fold and captured by Mouse Anti-Human BPIFB1 Monoclonal Antibody (MAB101952) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human BPIFB1 Monoclonal Antibody (Catalog # MAB101951) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BPIFB1

BPIFB1 (BPI fold-containing family B member 1), also named LPLUNC1, is a member of the BPI-fold (BPIF) containing/Plunc (palate, lung, and nasal epithelium clone) superfamily of putative innate defense molecules which are predominantly expressed in regions of the oral cavity, nasopharynx and upper respiratory tract (1, 2). BPIF proteins exist as two subgroups, BPIFA (formally SPLUNCs) and BPIFB (formally LPLUNCs) (1, 3). BPIFA proteins have structural homology to the N-terminal domain of BPI whereas BPIFB proteins have structural homology to both domains of BPI (2). Human BPIFB1 cDNA encodes a 484 amino acid (aa) precursor protein with a putative 21 aa signal peptide and a 463 aa mature chain. The mature human BPIFB1 shares approximately 57% and 56% aa sequence identity with mouse and rat BPIFB1, respectively. BPIF proteins appear to exhibit distinct tissue and cell specific expression patterns with various family members, being localized to a number of glandular structures within the upper respiratory tract, nasopharyngeal regions and oral cavity where they are secreted from these tissues and are found in high levels in saliva and nasal and respiratory lining fluids (2). BPIFB1 plays a role in diverse functions, including neutralizing endotoxin (LPS) in septic shock patients, inhibition of endothelial cell growth, dendritic cell maturation, as an anti-angiogenic, chemoattractant or opsonization agent (2). Although less characterized as BPIFA1, BPIFB1 may also function as an innate immune molecule sensing and responding to Gram-negative bacteria (4). BPIFA1 and BPIFB1 expression was increased in late stage chronic obstructive pulmonary disease (COPD) patients, and elevated levels correlate with disease severity (5). BPIFB1 is also upregulated in cystic fibrosis (CF) lung disease and may play a role in the pathogenesis of the disease (6).

References
  1. Bingle, C. D. and C. J. Craven (2002) Hum. Mol. Genet. 11:937.
  2. Alves, D. B. et al. (2017) Braz. Oral Res. 31:e6.
  3. Bingle, L. et al. (2012) Histochem. Cell Biol. 138:749.
  4. Balakrishnan, A. et al. (2013) Innate Immun. 19:339.
  5. De Smet, E. G. et al. (2017) Int. J. Chron. Obstruct. Pulmon. Dis. 13:11.
  6. Saferali, A. et al. (2015) Am. J. Respir. Cell. Mol. Biol. 53:607.
Long Name
BPI Fold Containing Family B Member 1
Entrez Gene IDs
92747 (Human); 228801 (Mouse); 499926 (Rat)
Alternate Names
bA49G10.6; BPIFB1; C20orf114; chromosome 20 open reading frame 114; dJ1187J4.1; long palate, lung and nasal epithelium carcinoma associated 1; long palate, lung and nasal epithelium carcinoma-associated protein 1; LPLUNC1; MGC14597; VEMSGP; Von Ebner minor salivary gland protein

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human BPIFB1 Antibody

There are currently no reviews for this product. Be the first to review Human BPIFB1 Antibody and earn rewards!

Have you used Human BPIFB1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review