Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1023923
View all BPIFB1 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human BPIFB1
Thr22-Gln484
Accession # Q8TDL5

Specificity

Detects human BPIFB1 in sandwich ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human BPIFB1 Antibody

Human BPIFB1 ELISA Standard Curve.

Recombinant Human BPIFB1 protein was serially diluted 2-fold and captured by Mouse Anti-Human BPIFB1 Monoclonal Antibody (Catalog # MAB101952) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human BPIFB1 Monoclonal Antibody (MAB101951) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

BPIFB1 in Human Lung.

BPIFB1 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human BPIFB1 Monoclonal Antibody (Catalog # MAB101952) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human BPIFB1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human BPIFB1 Monoclonal Antibody (Catalog # MAB101951).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BPIFB1

BPIFB1 (BPI fold-containing family B member 1), also named LPLUNC1, is a member of the BPI-fold (BPIF) containing/Plunc (palate, lung, and nasal epithelium clone) superfamily of putative innate defense molecules which are predominantly expressed in regions of the oral cavity, nasopharynx and upper respiratory tract (1, 2). BPIF proteins exist as two subgroups, BPIFA (formally SPLUNCs) and BPIFB (formally LPLUNCs) (1, 3). BPIFA proteins have structural homology to the N-terminal domain of BPI whereas BPIFB proteins have structural homology to both domains of BPI (2). Human BPIFB1 cDNA encodes a 484 amino acid (aa) precursor protein with a putative 21 aa signal peptide and a 463 aa mature chain. The mature human BPIFB1 shares approximately 57% and 56% aa sequence identity with mouse and rat BPIFB1, respectively. BPIF proteins appear to exhibit distinct tissue and cell specific expression patterns with various family members, being localized to a number of glandular structures within the upper respiratory tract, nasopharyngeal regions and oral cavity where they are secreted from these tissues and are found in high levels in saliva and nasal and respiratory lining fluids (2). BPIFB1 plays a role in diverse functions, including neutralizing endotoxin (LPS) in septic shock patients, inhibition of endothelial cell growth, dendritic cell maturation, as an anti-angiogenic, chemoattractant or opsonization agent (2). Although less characterized as BPIFA1, BPIFB1 may also function as an innate immune molecule sensing and responding to Gram-negative bacteria (4). BPIFA1 and BPIFB1 expression was increased in late stage chronic obstructive pulmonary disease (COPD) patients, and elevated levels correlate with disease severity (5). BPIFB1 is also upregulated in cystic fibrosis (CF) lung disease and may play a role in the pathogenesis of the disease (6).

References

  1. Bingle, C. D. and C. J. Craven (2002) Hum. Mol. Genet. 11:937.
  2. Alves, D. B. et al. (2017) Braz. Oral Res. 31:e6.
  3. Bingle, L. et al. (2012) Histochem. Cell Biol. 138:749.
  4. Balakrishnan, A. et al. (2013) Innate Immun. 19:339.
  5. De Smet, E. G. et al. (2017) Int. J. Chron. Obstruct. Pulmon. Dis. 13:11.
  6. Saferali, A. et al. (2015) Am. J. Respir. Cell. Mol. Biol. 53:607.

Long Name

BPI Fold Containing Family B Member 1

Alternate Names

C20orf114, LPLUNC1, VEMSGP

Entrez Gene IDs

92747 (Human); 228801 (Mouse); 499926 (Rat)

Gene Symbol

BPIFB1

UniProt

Q8TDL5

Additional BPIFB1 Products

Product Documents for Human BPIFB1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BPIFB1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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Protocols

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