Human BTLA Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB33542-100
MAB33542-SP
Human BTLA Antibody in Flow Cytometry
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Human BTLA Antibody Summary

Species Reactivity
Human
Specificity
Detects human BTLA in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2161A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line, NS0-derived human BTLA
Ile25-Ser150
Accession # AAP44003
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of BTLA in Human PBMC by Flow Cytometry. View Larger

Detection of BTLA in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Rabbit Anti-Human BTLA Monoclonal Antibody (Catalog # MAB33542) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Mouse anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTLA

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell co-stimulatory molecules (1-3). Mature human BTLA contains a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain. The two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail transmit inhibitory signaling (4-5). The ECD of human BTLA shares 42% and 44% aa identity with that of mouse and rat BTLA, respectively. A splice variant lacking the transmembrane domain has been reported (6). Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers (7). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (8). BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells (9). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells (9). BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR cross-linking (10). Under the control of ROR gamma t and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma δT cells (11). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha (12).

References
  1. Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671.
  2. Croft, M. (2005) Trends. Immunol. 26:292.
  3. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
  4. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
  5. Chemnitz, J.M. et al. (2006) J. Immunol. 176:6603.
  6. Han, P. et al. (2004) J. Immunol. 172 :5931.
  7. Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
  8. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  9. Hurchla, M.A. et al. (2005) J. Immunol. 174:3377.
  10. Krieg, C. et al. (2007) Nat. Immunol. 8:162.
  11. Bekiaris, V. et al. (2013) Immunity 39:1082.
  12. Cai G and Freeman GJ, (2009) Immunol Rev. 229:244
Long Name
B- And T-Lymphocyte Attenuator
Entrez Gene IDs
151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)
Alternate Names
B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; CD272 antigen; CD272; FLJ16065; MGC129743

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