Human c‑Rel Antibody

Name: Human c‑Rel Antibody
Brand: R&D Systems
SKU: MAB4606
Price: 369 USD
Availability: InStock

R&D Systems | Catalog # MAB4606

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 321422

View all c-Rel Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human c-Rel
Met1-Val619
Accession # NP_002899

Specificity

Detects human c-Rel in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human c‑Rel Antibody

Detection of Human c-Rel by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human c-Rel Monoclonal Antibody (Catalog # MAB4606) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for c-Rel at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

c‑Rel in Human PBMCs.

c-Rel was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Rat Anti-Human c-Rel Monoclonal Antibody (Catalog # MAB4606) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with nuclear stain (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human c‑Rel Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Western Blot

0.1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: c-Rel

c-Rel/Rel, the cellular counterpart of the v-Rel oncogene of the avian reticuloendotheliosis retrovirus, is a 85 kDa class II member of the Rel/NK-kappa B family of transcription factors. All Rel family members contain a RHD (Rel homology domain) that is involved in dimerization, DNA and I kappa B binding, and nuclear localization. Class II members contain an additional C-terminal transcriptional activation segment. Rel both homodimerizes and heterodimerizes with multiple family members. Following dimerization, Rel complexes initiate transcription by acting on decameric DNA motifs termed kappa B binding sites. c-Rel activity is regulated by phosphorylation and ubiquitination. The important role of c-Rel in B-cell development, growth, and survival is well documented. c-Rel is also involved in responses to auto-antigens, allo-antigens, allergens and pathogens and may contribute to the development of certain human lymphoid cell cancers.