Human C5L2/GPR77 Antibody

Catalog # Availability Size / Price Qty
MAB10254-100
MAB10254-SP
Human C5L2/GPR77 Antibody in Flow Cytometry
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human C5L2/GPR77 Antibody Summary

Species Reactivity
Human
Specificity
Detects human C5L2/GPR77 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 650918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human C5L2/GPR77
Met1-Val337
Accession # Q9P296
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of C5L2/GPR77 in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP by Flow Cytometry. View Larger

Detection of C5L2/GPR77 in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) C5L2/GPR77 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human C5L2/GPR77 Monoclonal Antibody (Catalog # MAB10254) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C5L2/GPR77

C5a anaphylatoxin chemotactic receptor C5a2, also known as C5L2 or GPR77, is a G protein-coupled receptor that in humans is encoded by the C5AR2. C5a2 is the receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides generated during the complement cascade that participate in host defense.

Long Name
C5a Anaphylatoxin Chemotactic Receptor C5L2
Entrez Gene IDs
27202 (Human); 319430 (Mouse); 445269 (Rat)
Alternate Names
C5a anaphylatoxin chemotactic receptor C5L2; C5L2; C5L2G-protein coupled receptor 77; G protein-coupled receptor 77; G protein-coupled receptor C5L2; GPF77; GPR77

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human C5L2/GPR77 Antibody

There are currently no reviews for this product. Be the first to review Human C5L2/GPR77 Antibody and earn rewards!

Have you used Human C5L2/GPR77 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review