Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 650918
View all C5L2/GPR77 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human C5L2/GPR77
Met1-Val337
Accession # Q9P296

Specificity

Detects human C5L2/GPR77 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human C5L2/GPR77 Antibody

Detection of C5L2/GPR77 antibody in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP antibody by Flow Cytometry.

Detection of C5L2/GPR77 in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) C5L2/GPR77 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human C5L2/GPR77 Monoclonal Antibody (Catalog # MAB10254) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Human C5L2/GPR77 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C5L2/GPR77

C5a anaphylatoxin chemotactic receptor C5a2, also known as C5L2 or GPR77, is a G protein-coupled receptor that in humans is encoded by the C5AR2. C5a2 is the receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides generated during the complement cascade that participate in host defense.

Long Name

C5a Anaphylatoxin Chemotactic Receptor C5L2

Alternate Names

GPR77

Entrez Gene IDs

27202 (Human); 319430 (Mouse); 445269 (Rat)

Gene Symbol

C5AR2

UniProt

Q9P296

Additional C5L2/GPR77 Products

Product Documents for Human C5L2/GPR77 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human C5L2/GPR77 Antibody

For research use only

Citations for Human C5L2/GPR77 Antibody

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