Human C5L2/GPR77 Antibody

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Detection of C5L2/GPR77 in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP by Flow Cytometry.
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Product Details
Citations (1)
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Human C5L2/GPR77 Antibody Summary

Species Reactivity
Detects human C5L2/GPR77 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 650918
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human C5L2/GPR77
Accession # Q9P296
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of C5L2/GPR77 antibody in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP antibody by Flow Cytometry. View Larger

Detection of C5L2/GPR77 in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) C5L2/GPR77 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human C5L2/GPR77 Monoclonal Antibody (Catalog # MAB10254) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C5L2/GPR77

C5a anaphylatoxin chemotactic receptor C5a2, also known as C5L2 or GPR77, is a G protein-coupled receptor that in humans is encoded by the C5AR2. C5a2 is the receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides generated during the complement cascade that participate in host defense.

Long Name
C5a Anaphylatoxin Chemotactic Receptor C5L2
Entrez Gene IDs
27202 (Human); 319430 (Mouse); 445269 (Rat)
Alternate Names
C5a anaphylatoxin chemotactic receptor C5L2; C5L2; C5L2G-protein coupled receptor 77; G protein-coupled receptor 77; G protein-coupled receptor C5L2; GPF77; GPR77

Product Datasheets

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Citation for Human C5L2/GPR77 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cancer-Associated Fibroblasts Promote Tumor Aggressiveness in Head and Neck Cancer through Chemokine Ligand 11 and C-C Motif Chemokine Receptor 3 Signaling Circuit
    Authors: WY Huang, YS Lin, YC Lin, S Nieh, YM Chang, TY Lee, SF Chen, KD Yang
    Cancers, 2022-06-27;14(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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