Human Cadherin-6/KCAD Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Cadherin-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Cadherin-8, rhCadherin-11, and rhCadherin-12 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Cadherin-6
    Thr22-Ala615
    Accession # P55285
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Cadherin‑6/KCAD Fc Chimera (Catalog # 2715-CA)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Cadherin‑6/KCAD in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Sheep Anti-Human Cadherin‑6/KCAD Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2715, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Immunocytochemistry
Cadherin‑6/KCAD in MG‑63 Human Cell Line. Cadherin‑6/KCAD was detected in immersion fixed MG‑63 human osteosarcoma cell line using Sheep Anti-Human Cadherin‑6/KCAD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2715) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cadherin-6/KCAD

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-to-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin‑6, also known as KCAD or K-cadherin, is a classical cadherin of 110-120 kD that has at least one full length and two alternate splice forms ranging in size from 105‑120 kDa (3). Human cadherin-6 is synthesized as a 790 amino acid (aa) type I transmembrane glycoprotein that contains a 18 aa signal peptide, a 35 aa prosequence, a 562 aa extracellular region, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (4, 5). There are five EC cadherin domains that are approximately 110 aa in length. This pattern is consistent with classical cadherin family molecules that are modular in their extracellular region and mediate calcium-dependent cell-to-cell adhesion through their Ca++-binding repeats (2). Due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat, Cadherin-6 can be further classified as a type II classical cadherin (4). One cadherin-6 splice variant (termed 6/2) shows a 9 aa substitution for the 94 aa that span residues 283 to 376 of the full-length extracellular region (3). A second splice variant shows a 36 aa substitution for the C-terminal 163 aa of the transmembrane and cytoplasmic region (6). The extracellular region of human cadherin-6 is 98% aa identical to rat cadherin-6 extracellular region, plus 60% and 58% aa identical to the extracellular regions of human cadherin 8 and 11, respectively. Cadherin-6 has high expression in kidney, brain, and cerebellum, and low expression in lung, pancreas, gastric mucosa, and cytotrophoblasts (4, 5, 7-9). Cadherin-6 is also found in renal, lung, and ovarian carcinoma (7, 10). As a classic cadherin, cadherin-6 will form homodimers and promote intercellular adhesion with itself and possibly, cadherin-9 and -14 (4, 11).

  • References:
    1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
    2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
    3. Mbalaviele, G. et al. (1998) J. Cell Biol. 141:1467.
    4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
    5. Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
    6. GenBank Accession # P55285.
    7. Xiang Y.Y. et al. (1994) Cancer Res. 54:3034.
    8. Marthiens V. et al. (2002) Mol. Cell Neurosci. 20:458.
    9. MacCalman C.D. et al. (1998) Am J Reprod. Immunol. 39:96.
    10. Sella, G.C. et al. (2001) Cancer Res. 61:6977.
    11. Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
  • Entrez Gene IDs:
    1004 (Human)
  • Alternate Names:
    cadherin 6, type 2, K-cadherin (fetal kidney); cadherin, fetal kidney; Cadherin6; Cadherin-6; CDH6; KCAD; K-cadherin (fetal kidney); K-Cadherin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Cadherin 6 has a functional role in platelet aggregation and thrombus formation.
    Authors: Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D
    Arterioscler. Thromb. Vasc. Biol., 2012;32(7):1724-31.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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