Human CaM Kinase IIδ Antibody Summary
Accession # Q13557
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CaM Kinase IIδ in Human Brain. CaM Kinase IIδ was detected in immersion fixed paraffin-embedded sections of human brain (cerebral cortex) using 15 µg/mL Sheep Anti-Human CaM Kinase IIδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human CaM Kinase IIδ by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, MDA‑MB‑468 human breast cancer cell line, and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CaM Kinase IIδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase IIδ at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CaM Kinase II delta
Calcium/calmodulin-dependent protein kinase type II (CaMKII) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. Each CaMKII protein assembles into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKIIδ expression is ubiquitous, and has been identified as the predominant isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.
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Cell and Tissue Staining Kits
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