Human CaM Kinase IIδ Antibody

Catalog # Availability Size / Price Qty
AF4176
AF4176-SP
CaM Kinase IIδ in Human Brain.
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Human CaM Kinase IIδ Antibody Summary

Species Reactivity
Human
Specificity
Detects human CaM Kinase IIδ isoforms. No cross-reactivity with recombinant human CaM Kinase II alpha, CaM Kinase II beta, or CaM Kinase II gamma is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CaM Kinase IIδ
Ala402-Lys478
Accession # Q13557
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry CaM Kinase IIδ in Human Brain. View Larger

CaM Kinase IIδ in Human Brain. CaM Kinase IIδ was detected in immersion fixed paraffin-embedded sections of human brain (cerebral cortex) using 15 µg/mL Sheep Anti-Human CaM Kinase IIδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human CaM Kinase IIδ by Western Blot. View Larger

Detection of Human CaM Kinase IIδ by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, MDA‑MB‑468 human breast cancer cell line, and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CaM Kinase IIδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase IIδ at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CaM Kinase II delta

Calcium/calmodulin-dependent protein kinase type II (CaMKII) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. Each CaMKII protein assembles into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKIIδ expression is ubiquitous, and has been identified as the predominant isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.

Long Name
Calcium/calmodulin-dependent Protein Kinase II delta Subunit
Entrez Gene IDs
817 (Human); 108058 (Mouse); 24246 (Rat)
Alternate Names
calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase type II delta chain; calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II delta subunit; CaM Kinase II delta; CaM kinase II subunit delta; CAMK2D; CAMKD; CaMK-II delta subunit; CaMK-II subunit delta; CaM-kinase II delta chain; DKFZp686G23119; DKFZp686I2288; EC 2.7.11; EC 2.7.11.17; MGC44911

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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