Detects human and canine IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant feline IL-23 p19 is observed and approximately 40% cross-reactivity with recombinant mouse IL-23 p19 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant canine IL-23 p19 Arg23-Pro193 Accession # XP_538231
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Canine IL‑23 p19 by Western Blot. Western blot shows lysates of human mature dendritic cells (LPS activated), CLL‑1390 canine leukocytic round cell neoplasia cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Canine IL‑23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6250) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL‑23 p19 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IL-23 p19 (Interleukin 23 p19; also IL-23 alpha) is an 18-20 kDa (estimated) member of the IL-6 superfamily of molecules. It is disulfide-bonded to p40 to form IL-23. IL-23 is secreted by immune-related cell types such as keratinocytes, dendritic cells, macrophages, microglia and monocytes, often following TLR stimulation. It appears to drive Th17 cell development by inhibiting T-bet and FoxP3 production, and to reduce IL-12-mediated IFN-gamma production by CD4+, CD8+ and NK cells. Based on human, mature canine IL-23 p19 is 171 amino acids (aa) in length. It is a alpha -helical molecule that utilizes Cys77 to form an interchain disulfide bond with IL-12 p40. IL-23 p19 does not appear to be released unless dimerized to p40. There are no potential N-linked glycosylation sites on p19. Mature canine IL-23 p19 (aa 23-193) shares 88% aa identity with human IL-23 p19.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.