Human Caspase-10 (FLICE 2) Antibody

R&D Systems | Catalog # AF834

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Caspase-10 Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled human Caspase-10 (FLICE 2) synthetic peptide
KRTVWGAKQISATSLPTAC

Specificity

Detects human and mouse Caspase-10 (FLICE 2) precursor in Western blots. Sensitivity for detection of the small subunit is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Caspase-10 (FLICE 2) Antibody

Detection of Human Caspase-10 (FLICE 2), antibody by Western Blot.

Detection of Human Caspase‑10 (FLICE 2), by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Caspase-10 (FLICE 2) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF834) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Caspase-10 (FLICE 2), at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human Caspase-10 (FLICE 2) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caspase-10

Caspase-10 (Cysteine-aspartic acid protease 10, FLICE 2 and Mch4) is a member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on Pro-Caspases-3, -4, -6, -7, and -9. Human Pro-Caspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43  generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.

Alternate Names

CASP10, Caspase10, FLICE2, Mch4

Entrez Gene IDs

843 (Human)

Gene Symbol

CASP10

Additional Caspase-10 Products

Product Documents for Human Caspase-10 (FLICE 2) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Caspase-10 (FLICE 2) Antibody

For research use only

Citations for Human Caspase-10 (FLICE 2) Antibody

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail