Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Caspase-10 Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled human Caspase-10 (FLICE 2) synthetic peptide
KRTVWGAKQISATSLPTAC

Specificity

Detects human and mouse Caspase-10 (FLICE 2) precursor in Western blots. Sensitivity for detection of the small subunit is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Caspase‑10 (FLICE 2) Antibody

Detection of Human Caspase-10 (FLICE 2), antibody by Western Blot.

Detection of Human Caspase‑10 (FLICE 2), by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Caspase-10 (FLICE 2) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF834) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Caspase-10 (FLICE 2), at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human Caspase‑10 (FLICE 2) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caspase-10

Caspase-10 (Cysteine-aspartic acid protease 10, FLICE 2 and Mch4) is a member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on Pro-Caspases-3, -4, -6, -7, and -9. Human Pro-Caspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43  generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.

Alternate Names

CASP10, Caspase10, FLICE2, Mch4

Entrez Gene IDs

843 (Human)

Gene Symbol

CASP10

Additional Caspase-10 Products

Product Documents for Human Caspase‑10 (FLICE 2) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Caspase‑10 (FLICE 2) Antibody

For research use only

Citations for Human Caspase‑10 (FLICE 2) Antibody

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail