Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 814208
View all CCDC134 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CCDC134
Thr23-Leu229
Accession # Q9H6E4

Specificity

Detects human CCDC134 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse CCDC134 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CCDC134 Antibody

Detection of Human CCDC134 antibody by Western Blot.

Detection of Human CCDC134 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CCDC134 Monoclonal Antibody (Catalog # MAB7784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCDC134 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human CCDC134 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCDC134

Coiled-coil domain-containing protein 134 (CCDC134) is a widely expressed secreted glycoprotein of approximately 35 kDa. It contains a single coiled-coil domain (aa 196‑218). Human CCDC134 shares 90% aa sequence identity with mouse and rat CCDC134.

Long Name

Coiled-coil Domain Containing 134

Alternate Names

coiled-coil domain containing 134, coiled-coil domain-containing protein 134, dJ821D11.3, FLJ22349, MGC21013

Entrez Gene IDs

79879 (Human); 76457 (Mouse); 500909 (Rat)

Gene Symbol

CCDC134

UniProt

Q9H6E4 (Human)

Additional CCDC134 Products

Product Documents for Human CCDC134 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CCDC134 Antibody

For research use only

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