Human CCDC134 Antibody

Catalog # Availability Size / Price Qty
MAB7784
MAB7784-SP
Detection of Human CCDC134 by Western Blot.
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Human CCDC134 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCDC134 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse CCDC134 is observed.
Source
Monoclonal Mouse IgG2A Clone # 814208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CCDC134
Thr23-Leu229
Accession # Q9H6E4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human CCDC134 by Western Blot. View Larger

Detection of Human CCDC134 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CCDC134 Monoclonal Antibody (Catalog # MAB7784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCDC134 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCDC134

Coiled-coil domain-containing protein 134 (CCDC134) is a widely expressed secreted glycoprotein of approximately 35 kDa. It contains a single coiled-coil domain (aa 196‑218). Human CCDC134 shares 90% aa sequence identity with mouse and rat CCDC134.

Long Name
Coiled-coil Domain Containing 134
Entrez Gene IDs
79879 (Human); 76457 (Mouse); 500909 (Rat)
Alternate Names
CCDC134; coiled-coil domain containing 134; coiled-coil domain-containing protein 134; dJ821D11.3; FLJ22349; MGC21013

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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