Human CCR10 MAb (Clone 314305)

Catalog # Availability Size / Price Qty
MAB3478
MAB3478-SP
Detection of CCR10  in Human Blood Lymphocytes by Flow Cytometry.
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human CCR10 MAb (Clone 314305) Summary

Species Reactivity
Human
Specificity
Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 314305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with human CCR10
Gln8-Asn362
Accession # P46092
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR10  in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of CCR10 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Rat Anti-Human CCR10 Monoclonal Antibody (Catalog # MAB3478) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that function as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs
2826 (Human); 12777 (Mouse); 363682 (Rat)
Alternate Names
CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2

Product Datasheets

Citations for Human CCR10 MAb (Clone 314305)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells.
    Authors: McCully M, Collins P, Hughes T, Thomas C, Billen J, O'Donnell V, Moser B
    J Immunol, 2015;195(1):96-104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes.
    Authors: Duhen T, Campbell D
    J Immunol, 2014;193(1):120-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia.
    Authors: Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E
    PLoS ONE, 2014;9(8):e106008.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression.
    Authors: Yamanaka K, Dimitroff CJ, Fuhlbrigge RC, Kakeda M, Kurokawa I, Mizutani H, Kupper TS
    J. Allergy Clin. Immunol., 2007;121(1):148-157.e3.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  5. Microbe-specific unconventional T cells induce human neutrophil differentiation into antigen cross-presenting cells.
    Authors: Davey M, Morgan M, Liuzzi A, Tyler C, Khan M, Szakmany T, Hall J, Moser B, Eberl M
    J Immunol, 0;193(7):3704-3716.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CCR10 MAb (Clone 314305)

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human CCR10 MAb (Clone 314305)?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human CCR10 Antibody
By Anonymous on 06/28/2016
Application: CyTOF Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human