Human CCR7 Alexa Fluor® 594-conjugated Antibody

FAB197T Datasheet / COA / SDS

Clone 150503 was used by HLDA to establish CD designation

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FAB197T-025

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Detection of CCR7 in Human PBMCs by Flow Cytometry.

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Human CCR7 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR7.

Source

Monoclonal Mouse IgG_2A Clone # 150503

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CCR7 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR7 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB197T) or (B) Mouse IgG_2A Alexa Fluor 594 Isotype Control (Catalog # IC003T). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR7

CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Alternate Names

BLR2; BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7; CMKBR7chemokine (C-C) receptor 7; EBI1; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor