Human CCR7 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of CCR7 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR7 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB197T) or (B) Mouse IgG_2AAlexa Fluor 594 Isotype Control (Catalog # IC003T). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Product Datasheets

Related Research Areas

• Cancer Biomarkers
• CC Chemokines and Receptors
• Chemokine Receptors
• Chemokines and Receptors in Angiogenesis
• Chemokines and Receptors in Tfh Cell Differentiation and Function
• Cytokine and Growth Factor Receptors on VSMC
• Dendritic Cell Adhesion and Migration
• G Protein-Coupled Receptors in Immune Responses
• Regulatory T Cells (Tregs)
• T Cell Migration/Adhesion